SARS-CoV-2 NSP1 Alexa Fluor™ Plus 488‑conjugated Antibody

R&D Systems | Catalog # FAB10980AFP488

R&D Systems

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunohistochemistry, ELISA, Immunocytochemistry

Label

Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 1045410
View all NSP1 Primary Antibodies »

Product Specifications

Specificity

Detects Sars-CoV-2 NSP1 protein in ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for SARS-CoV-2 NSP1 Alexa Fluor™ Plus 488‑conjugated Antibody

Application
Recommended Usage

ELISA

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NSP1

Non-structural protein 1 (NSP1) is one of several functional proteins released by ORF1a-encoded protease cleavage of the pp1a and pp1ab replicase polyproteins expressed from the coronavirus (CoV) genome (1). The NSPs are involved in the replication and transcription of the viral RNA and not incorporated within the virion particles. Coronaviruses include various highly pathogenic strains such as SARS-CoV, MERS-CoV and SARS-CoV2 that have had significant impact on humans as well as strains that have negatively impacted livestock. NSP1, also known as the host shutoff factor, is a small 180 amino acid highly conserved protein among the first to be expressed following cell entry.  It is composed of an N-terminal domain, a linker region, and a C-terminal domain with a conserved KH motif that is required and sufficient for specific contacts with ribosomes (2, 3). The C-terminal domain binds tightly to and sterically occludes the entrance region of the mRNA channel in free 40S subunits, 43S pre-initiation complex, and empty, non-translating 80S ribosomes (2, 3) to inhibit translation. NSP1 blocks the innate immune response through suppression of host gene expression. By preventing translation of interferon and downstream signaling responses, it plays a key role in immune evasion (2, 4, 5). NSP1 also induces endonucleolytic cleavage of host mRNAs (6,7). Concomitant translation of more efficiently recognized untranslated regions (UTR) of viral mRNA along with resistance to endonucleolytic cleavage (7) is thought to lead to a switch to production of viral mRNA over host cell mRNA during an infection (3,8). Given the critical role NSP1 plays in virulence, it is an attractive target for small-molecule inhibition and vaccination development (9, 10).  

References

  1. Snijder, E.J. et al. (2016) Adv. Virus Res 96:59.
  2. Thoms, M. et al. (2020) Science 369:1249.
  3. Schubert, K. et al. (2020) Nat. Struct. Mol. Biol. 27:959.
  4. Narayanan, K. et al. (2008) J. Virol. 82:4471.
  5. Jauregui, A.R. et al. (2013) PLoS One. 8:e62416.
  6. Kamitani, W. et al. (2006) Proc. Natl. Acad. Sci. USA 103:12885.
  7. Huang, C. et al. (2011) PLoS Pathog. 7:e1002433.
  8. Hartenian, E. et al. (2020) J. Biol. Chem. 295:12910. 
  9. Zust, R. et al. (2007) PLoS Pathog. 3:e109.
  10. De Lima Menezes, G. and R.A. da Silva (2020) J. Biomol. Struct. Dyn. (In press). 

Long Name

Non-Structural Protein 1

UniProt

YP_009725297.1

Additional NSP1 Products

Product Documents for SARS-CoV-2 NSP1 Alexa Fluor™ Plus 488‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for SARS-CoV-2 NSP1 Alexa Fluor™ Plus 488‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

Related Research Areas

Infectious Diseases

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Protocols

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FAQs

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