SARS-CoV-2 nsp13 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-07964

Novus Biologicals
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Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SARS-CoV-2 nsp13 Primary Antibodies »

Product Specifications

Immunogen

Recombinant SARS-CoV-2 NSP13(Ala5325-Gln5925)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SARS-CoV-2 nsp13 Antibody - Azide and BSA Free

Western Blot: SARS-CoV-2 nsp13 AntibodyAzide and BSA Free [NBP3-07964]

Western Blot: SARS-CoV-2 nsp13 AntibodyAzide and BSA Free [NBP3-07964]

Western Blot: SARS-CoV-2 nsp13 Antibody [NBP3-07964] - Various lysates were subjected to SDS-PAGE followed by western blot with SARS-CoV-2 (2019-nCoV) Nsp13 Protein antibody at dilution of 1:1000.

Applications for SARS-CoV-2 nsp13 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:4000-1:8000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 nsp13

SARS-CoV-2 Nonstructural Protein 13 (NSP13) is one of the sixteen nonstructural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1). SARS-CoV-2 NSP13 is 601 amino acids (aa) with a theoretical molecular weight of 66.9 kDa (1-3). The amino acid sequence alignment of SARS-CoV and SARS-CoV-2's NSP13 has 99.8% sequence identity and 100% sequence similarity, with only one amino acid residue difference between the two proteins (1). NSP13 is suggested to have helicase activity and unwinds RNA (1,3). Furthermore, NSP13 is suggested to interact with NSP12 and through protein-protein interactions, NSP12 enhances NSP13's helicase activity (1,3,4). Additionally, a SARS-CoV-2 interactome study reveals NSP13's involvement in several processes including transcriptional regulation, Golgi organization, protein kinase A signaling, and centrosome complex formation (2). Given its role in centrosome organization, NSP13 is a possible molecular link for the loss of smell, called anosmia, due to the olfactory cilia defects that can occur because of COVID-19 infection (5). NSP13 also interacts with innate immune pathways including the interferon pathway and NF-kappaB pathway (2).

References

1. Yoshimoto F. K. (2020). The Proteins of Severe Acute Respiratory Syndrome Coronavirus-2 (SARS CoV-2 or n-COV19), the Cause of COVID-19. The Protein Journal. https://doi.org/10.1007/s10930-020-09901-4

2. Gordon, D. E., Jang, G. M., Bouhaddou, M., Xu, J., Obernier, K., White, K. M., O'Meara, M. J., Rezelj, V. V., Guo, J. Z., Swaney, D. L., Tummino, T. A., Huttenhain, R., Kaake, R. M., Richards, A. L., Tutuncuoglu, B., Foussard, H., Batra, J., Haas, K., Modak, M., Kim, M.,... Krogan, N. J. (2020). A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature. https://doi.org/10.1038/s41586-020-2286-9

3. Qiu, Y., & Xu, K. (2020). Functional studies of the coronavirus nonstructural proteins. STEMedicine. https://doi.org/10.37175/stemedicine.v1i2.39

4. Romano, M., Ruggiero, A., Squeglia, F., Maga, G., & Berisio, R. (2020). A Structural View of SARS-CoV-2 RNA Replication Machinery: RNA Synthesis, Proofreading and Final Capping. Cells. https://doi.org/10.3390/cells9051267

5. Li, W., Li, M., & Ou, G. (2020). COVID-19, cilia, and smell. The FEBS journal. https://doi.org/10.1111/febs.15491

Alternate Names

helicase, Non-structural protein 13

Gene Symbol

ORF1ab

Additional SARS-CoV-2 nsp13 Products

Product Documents for SARS-CoV-2 nsp13 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SARS-CoV-2 nsp13 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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