SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-07066

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

SARS-CoV-2

Applications

Immunohistochemistry, ELISA, Neutralization

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 2165 expressed in HEK293

Format

Azide and BSA Free
View all SARS-CoV-2 Spike RBD Primary Antibodies »

Product Specifications

Immunogen

Sequenced from human survivors of COVID-19 (SARS-CoV-2). The spike RBD is expressed on the surface of SARS-CoV-2.

Specificity

This antibody specifically targets an epitope on the SARS-CoV-2 spike protein receptor-binding domain (RBD). This antibody does not cross-react with the N-terminal domain (NTD) of the spike protein or the RBD of SARS-CoV.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Scientific Data Images for SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free

Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free [NBP3-07066]

Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free [NBP3-07066]

Immunohistochemistry: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody (clone 2165) IHC data using infected cell pellets.
Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free [NBP3-07066]

Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free [NBP3-07066]

Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - SARS-CoV-2 Spike RBD Antibody to the Receptor Binding Domain (RBD) (Clone 2165). Antibody binds to RBD and blocks binding to the ACE2 receptor on coated plates using a neutralization ELISA assay.
Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free [NBP3-07066]

Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free [NBP3-07066]

Neutralization: SARS-CoV-2 Spike RBD Antibody (2165) [NBP3-07066] - Coating: Purified Recombinant SARS-CoV-2 Spike RBD, concentration of 1 ul/well, 100 ul/well overnight 2-8C. Detection: SARS-CoV-2 Spike RBD (Clone 2165) conjugated to HRP was serially diluted starting at 25 ng/ml down to 0.23 ng/ml, 100 ul/well for 1 hour at 37C. Substrate: TMB, 100 ul/well for 15 min. at room temperature followed by 450 nm stop solution, 50ul/well.

Applications for SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.01M PBS (pH 7.2 - 7.4), 150mM NaCl

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SARS-CoV-2 Spike RBD

The SARS-CoV-2 Spike protein is one of the four major structural proteins of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19 (1,2). The spike protein is the largest of the structural proteins, which also include the membrane (M), envelope (E), and nucleocapsid (N) proteins (1,2). The SARS-CoV-2 spike protein is a 1273 amino acid (aa) heterotrimeric class I fusion protein with each monomer having a theoretical molecular weight of approximately 180 kDa (1). The club-shaped spike protein contains several functional regions and domains including the S1 globular head region which contains the N-terminal receptor-binding domain (RBD) and the S2 stem region that contains the C-terminal fusion domain, two heptad regions, a transmembrane domain, and a cytoplasmic tail (1,2). The viral spike protein is critical for attachment of the virus with the host cell, resulting in fusion and virus entry into the cell (1,2). More specifically, the RBD of the spike protein is responsible for binding to the cell surface receptor angiotensin converting enzyme 2 (ACE2) (1,2). This spike-ACE2 interaction results in a conformational change permitting furin cleavage between the S1 and S2 domains and then cleavage at S2' by TMPRRS2, or another protease, allowing membrane fusion (1,2).

Given the critical role of the spike protein RBD in the interaction with the ACE2 receptor and viral entry, a number of neutralizing antibodies against the RBD have been developed as potential therapeutics for treating COVID-19 (3). These antibodies bind the RBD domain on the S1 subunit inhibiting the interaction with ACE2 (3). However, more studies need to be done as neutralizing antibodies can result in antibody-dependent enhancement, in which the viral entry and replication within the host cell is increased (4). One potential way to combat antibody-dependent enhancement is the use of nanobodies (4). Furthermore, there are currently several vaccine strategies that are in clinical trials, or recently federally approved, that utilize the spike protein in different forms (e.g. full length, S1 RBD, RBD-Fc, N-terminal) for protecting against SARS-CoV-2 infection (4,5). These vaccine strategies include DNA vaccines, viral vector-based vaccines, RNA vaccines, and subunit vaccines (4,5).

References

1. Pillay T. S. (2020). Gene of the month: the 2019-nCoV/SARS-CoV-2 novel coronavirus spike protein. Journal of Clinical Pathology. https://doi.org/10.1136/jclinpath-2020-206658

2. Malik Y. A. (2020). Properties of Coronavirus and SARS-CoV-2. The Malaysian Journal of Pathology.

3. Ho M. (2020). Perspectives on the development of neutralizing antibodies against SARS-CoV-2. Antibody Therapeutics. https://doi.org/10.1093/abt/tbaa009

4. Samrat, S. K., Tharappel, A. M., Li, Z., & Li, H. (2020). Prospect of SARS-CoV-2 spike protein: Potential role in vaccine and therapeutic development. Virus Research. https://doi.org/10.1016/j.virusres.2020.198141

5. Sternberg, A., & Naujokat, C. (2020). Structural features of coronavirus SARS-CoV-2 spike protein: Targets for vaccination. Life Sciences. https://doi.org/10.1016/j.lfs.2020.118056

Alternate Names

2019-nCoV Peplomer protein, 2019-nCoV S Protein, 2019-nCoV Spike, COVID-19 Spike, E2, Human coronavirus spike glycoprotein, Peplomer protein, S glycoprotein, S protein, SARS-CoV-2, SARS-COV-2 E2, SARS-COV-2 Peplomer protein, SARS-COV-2 S protein, SARS-COV-2 Spike glycoprotein, SARSCOV2 Spike protein, Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein, Spike, Spike glycoprotein, Spike S1, Spike S1 RBD, surface glycoprotein

Gene Symbol

S

Additional SARS-CoV-2 Spike RBD Products

Product Documents for SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SARS-CoV-2 Spike RBD Antibody (2165) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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