SB 203580 hydrochloride

Tocris Bioscience | Catalog # 1402

Selective inhibitor of p38 MAPK; water-soluble
Tocris Bioscience

Key Product Details

Description

Selective inhibitor of p38 MAPK; water-soluble
View all p38 Inhibitors »

Product Description

SB 203580 hydrochloride is a water-soluble salt of SB 203580 (Cat. No. 1202). Selective inhibitor of p38 mitogen-activated protein kinase (IC50 values are 50 and 500 nM for SAPK2a/p38 and SAPK2b/p38β2 respectively). Displays 100-500-fold selectivity over LCK, GSK3β and PKBα. Shown to inhibit interleukin-2-induced T cell proliferation, cyclooxygenase-1 and -2, and thromboxane synthase.

Product Specifications for SB 203580 hydrochloride

Molecular Weight

413.89

Formula

C21H16FN3OS.HCl

Storage

Store at -20°C

Purity

≥98% (HPLC)

Chemical Name

4-[5-(4-Fluorophenyl)-2-[4-(methylsulphonyl)phenyl]-1H-imidazol-4-yl]pyridine hydrochloride

CAS Number

869185-85-3

PubChem ID

16760644

InChI Key

WOSGGXINSLMASH-UHFFFAOYSA-N

SMILES

Cl.CS(=O)C1=CC=C(C=C1)C1=NC(=C(N1)C1=CC=NC=C1)C1=CC=C(F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
water 10.35 25

Preparing Stock Solutions for SB 203580 hydrochloride

The following data is based on the product molecular weight 413.89.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 9.66 mL 48.32 mL 96.64 mL
1.25 mM 1.93 mL 9.66 mL 19.33 mL
2.5 mM 0.97 mL 4.83 mL 9.66 mL
12.5 mM 0.19 mL 0.97 mL 1.93 mL

Calculators

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 27 publications citing the usage of this product.

Product Documents for SB 203580 hydrochloride

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SB 203580 hydrochloride

For research use only

Citations for SB 203580 hydrochloride

Customer Reviews for SB 203580 hydrochloride (4)

4.8 out of 5
4 Customer Ratings
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4 Stars
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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Generation of EPS-blastoids
    Name: Anonymous
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: EPS
    Verified Customer | Posted 02/27/2020
    10uM
    SB 203580 hydrochloride 1402
  • p38 MAPK pathway does not influence the LPA induced AMPK phosphorylation in microglia cells.
    Name: lisha joshi
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: BV2 microglia cells
    Verified Customer | Posted 06/18/2019
    We investigate the effect of LPA on microglia metabolic states. Trying to unravel possible pathways that maybe involved in this process, we used LPAR inhibitors and downstream inhibitors of MAPKs pathway. SB 203580 was used at a concentration of 10µM yet couldn't inhibit the expression of pAMPK. However the inhibitor was well tolerated by the cells.
    SB 203580 hydrochloride 1402
  • SB 203580 ameliorates LPA-induced COX-2 expression
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: Osteosarcoma
    Verified Customer | Posted 12/13/2018
    MG-63 cells were pre-incubated with 10 µM SB 203580 for 30 min followed by treatment with 10 µM LPA for 3 h to follow COX-2 expression using Western blot. SB 203580 inhibited COX-2 induction by LPA.
    SB 203580 hydrochloride 1402
  • SB 203580 blunts 15d-PGJ2 induced pp38 expression
    Name: Chintan Koyani
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: HL-1 cardiomyocytes
    Verified Customer | Posted 10/13/2018
    HL-1 cells were incubated with p38 MAPK inhibitor (10 µM SB203580, 25 µM PD169316) or p42/44 MAPK kinase inhibitor (25 µM PD98059) for 30 min prior to 15dPGJ2 treatment (15 µM, 1 h). Immunoreactive bands for pp38 and pp42/44 MAPK expressions were followed by Western blot. SB203580 completely inhibited 15d-PGJ2 induced p38 phosphorylation (shown in Image).
    SB 203580 hydrochloride 1402

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