SB 203580 hydrochloride
Chemical Name: 4-[5-(4-Fluorophenyl)-2-[4-(methylsulphonyl)phenyl]-1H-imidazol-4-yl]pyridine hydrochloride
Biological ActivitySB 203580 hydrochloride is a water-soluble salt of SB 203580 (Cat. No. 1202). Selective inhibitor of p38 mitogen-activated protein kinase (IC50 values are 50 and 500 nM for SAPK2a/p38 and SAPK2b/p38β2 respectively). Displays 100-500-fold selectivity over LCK, GSK3β and PKBα. Shown to inhibit interleukin-2-induced T cell proliferation, cyclooxygenase-1 and -2, and thromboxane synthase.
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Specificity and mechanism of action of some commonly used protein kinase inhibitors.
Davies et al.
SB 203580 is a specific inhibitor of a MAP kinase homologue which is stimulated by cellular stresses and interleukin-1.
Cuenda et al.
FEBS Lett., 1995;364:229
Direct inhibition of cyclooxygenase-1 and -2 by the kinase inhibitors SB 203580 and PD 98059.
Borsch-Haubold et al.
Role for p38 mitogen-activated protein kinase in platelet aggregation caused by collagen on a thromboxane analogue.
Saklatvala et al.
Citations for SB 203580 hydrochloride
The citations listed below are publications that use Tocris products. Selected citations for SB 203580 hydrochloride include:
11 Citations: Showing 1 - 10
Generation of blastocyst-like structures from mouse embryonic and adult cell cultures.
Authors: Li Et al.
Elevated sodium chloride drives type I IF. signaling in macrophages and increases antiviral resistance.
Authors: Zhang Et al.
J Biol Chem 2018;293:1030
Establishment of mouse expanded potential stem cells.
Authors: Yang Et al.
Chronic hypoxia alters fetal cerebrovascular responses to endothelin-1.
Authors: Silpanisong Et al.
Am J Physiol Cell Physiol 2017;313:C207
Loss of fibronectin from the aged stem cell niche affects the regenerative capacity of skeletal muscle in mice.
Authors: Lukjanenko Et al.
Nat Med 2016;22:897
Tissue factor/factor VIIa signalling promotes cytokine-induced beta cell death and impairs glucose-stimulated Ins secretion from human pancreatic islets.
Authors: Edén Et al.
Transient receptor potential vanilloid type 1 channel may modulate opioid reward.
Authors: Nguyen Et al.
An acetylcholinesterase inhibitor, eserine, induces long-term depression at CA3-CA1 synapses in the hippocampus of adult rats.
Authors: Mans Et al.
J Neurophysiol 2014;112:2388
Inhibition of stathmin1 accelerates the metastatic process.
Authors: Williams Et al.
Cancer Res 2012;72:5407
FGF1/p38 MAP kinase inhibitor therapy induces cardiomyocyte mitosis, reduces scarring, and rescues function after myocardial infarction.
Authors: Engel Et al.
Proc Natl Acad Sci U S A 2006;103:15546
Mechanisms underlying the relaxation response induced by bradykinin in the epithelium-intact guinea-pig trachea in vitro.
Authors: Schlemper Et al.
Br J Pharmacol 2005;145:740
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Reviews for SB 203580 hydrochloride
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We investigate the effect of LPA on microglia metabolic states. Trying to unravel possible pathways that maybe involved in this process, we used LPAR inhibitors and downstream inhibitors of MAPKs pathway. SB 203580 was used at a concentration of 10µM yet couldn't inhibit the expression of pAMPK. However the inhibitor was well tolerated by the cells.
MG-63 cells were pre-incubated with 10 µM SB 203580 for 30 min followed by treatment with 10 µM LPA for 3 h to follow COX-2 expression using Western blot. SB 203580 inhibited COX-2 induction by LPA.
HL-1 cells were incubated with p38 MAPK inhibitor (10 µM SB203580, 25 µMPD169316) or p42/44 MAPK kinase inhibitor (25 µM PD98059) for 30 min prior to 15dPGJ2 treatment (15 µM, 1 h). Immunoreactive bands for pp38 and pp42/44 MAPK expressions were followed by Western blot. SB203580 completely inhibited 15d-PGJ2 induced p38 phosphorylation (shown in Image).