SB 203580

Catalog # Availability Size / Price Qty
SB 203580 | CAS No. 152121-47-6 | p38 Inhibitors
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Description: Selective inhibitor of p38 MAPK

Chemical Name: 4-[5-(4-Fluorophenyl)-2-[4-(methylsulfonyl)phenyl]-1H-imidazol-4-yl]pyridine

Purity: ≥98%

Product Details
Citations (106)
Reviews (2)

Biological Activity

SB 203580 is a selective inhibitor of p38 MAPK (IC50 values are 50 and 500 nM for SAPK2a/p38 and SAPK2b/p38β2 respectively). Displays 100-500-fold selectivity over LCK, GSK-3β and PKBα. Shown to inhibit IL-2-induced T cell proliferation, cyclooxygenase-1 and -2, and thromboxane synthase. Enhances clonal growth of skin epithelial progenitor cells; stimulates neural stem cell (NSC) proliferation. SB 203580 is an essential component of medium for maintaining stem cells in naive pluripotent state and can be used to promote expansion of HSCs ex vivo.

Water-soluble Salt also available.

Technical Data

Soluble to 25 mM in DMSO
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Additional Information

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Citations for SB 203580

The citations listed below are publications that use Tocris products. Selected citations for SB 203580 include:

106 Citations: Showing 1 - 10

  1. Microglia Are Indispensable for Synaptic Plasticity in the Spinal Dorsal Horn and Chronic Pain.
    Authors: Zhou Et al.
    Cell Rep  2019;27:3844
  2. Osmotic induction of cyclooxygenase-2 in RPE cells: Stimulation of inflammasome activation.
    Authors: Messerschmidt Et al.
    Mol Vis  2019;25:329
  3. Scratching Counteracts IL-13 Signaling by Upregulating the Decoy Receptor IL-13Rα2 in Keratinocytes.
    Authors: Ulzii Et al.
    Int J Mol Sci  2019;20
  4. Whole Genome Microarray Analysis of DUSP4-Deletion Reveals A Novel Role for MAP Kinase Phosphatase-2 (MKP-2) in Macrophage Gene Expression and Function.
    Authors: Neamatallah Et al.
    Int J Mol Sci  2019;20
  5. Interleukin (IL)-22 from IL-20 Subfamily of Cytokines Induces Colonic Epithelial Cell Proliferation Predominantly through ERK1/2 Pathway.
    Authors: Moniruzzaman Et al.
    Int J Mol Sci  2019;20
  6. Combination of MetF. and p38 MAPK inhibitor, SB203580, reduced myocardial ischemia/reperfusion injury in non-obese type 2 diabetic Goto-Kakizaki rats.
    Authors: Sanit Et al.
    Exp Ther Med  2019;18:1701
  7. LRRK2 kinase plays a critical role in manganese-induced inflammation and apoptosis in microglia.
    Authors: Kim Et al.
    PLoS One  2019;14:e0210248
  8. HIV gp120 upregulates tonic inhibition through α5-containing GABAARs.
    Authors: green and Thayer Et al.
    Neuropharmacology  2019;149:161
  9. Interleukin-1β induces CXCR3-mediated chemotaxis to promote umbilical cord mesenchymal stem cell transendothelial migration.
    Authors: Guo Et al.
    Stem Cell Res Ther  2018;9:281
  10. Decreased Expression of TRPV4 Channels in HEI-OC1 Cells Induced by High Glucose Is Associated with Hearing Impairment.
    Authors: Xing Et al.
    Yonsei Med J  2018;59:1131
  11. Suppressive effects of berberine on atherosclerosis via downregulating visfatin expression and attenuating visfatin-induced endothelial dysfunction.
    Authors: Wan Et al.
    Int J Mol Med  2018;41:1939
  12. Differential effect of p38 and MK2 kinase inhibitors on the inflammatory and toxicity biomarkers in vitro.
    Authors: Singh Et al.
    Hum Exp Toxicol  2018;37:521
  13. Molecular mechanisms of IL-33-mediated stromal interactions in cancer metastasis.
    Authors: Andersson Et al.
    JCI Insight  2018;3
  14. The p38α Stress Kinase Suppresses Aneuploidy Tolerance by Inhibiting Hif-1α.
    Authors: Simões-Sousa Et al.
    Cell Rep  2018;25:749
  15. Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
    Authors: Kang Et al.
    Cell Rep  2018;25:2563
  16. Activator protein-1 contributes to the NaCl-induced expression of VEGF and PlGF in RPE cells.
    Authors: Kleiner Et al.
    Mol Vis  2018;24:647
  17. Capsaicin-induced TRIB3 upregulation promotes apoptosis in cancer cells.
    Authors: Lin Et al.
    Cancer Manag Res  2018;10:4237
  18. Prostaglandin E2-mediated adenosinergic effects on CD14+ cells: Self-amplifying immunosuppression in cancer.
    Authors: Al-Taei Et al.
    Oncoimmunology  2017;6:e1268308
  19. TRIB3 downregulation enhances doxorubicin-induced cytotoxicity in gastric cancer cells.
    Authors: Wu
    Arch Biochem Biophys  2017;622:26
  20. Derivation of Pluripotent Stem Cells with In Vivo Embryonic and Extraembryonic Potency.
    Authors: Yang Et al.
    Cell  2017;169:243
  21. Cannabinoid receptor 1 contributes to sprouted innervation in endometrial ectopic growth through mitogen-activated protein kinase activation.
    Authors: Han
    Brain Res  2017;1663:132
  22. Dynamic regulation of VEGF-inducible genes by an ERK/ERG/p300 transcriptional network.
    Authors: Fish Et al.
    Development  2017;144:2428
  23. The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages.
    Authors: Yang Et al.
    Cardiorenal Med  2016;7:11385
  24. Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.
    Authors: Chang Et al.
    PLoS One  2016;11:e0165715
  25. 18α-glycyrrhetinic acid lethality for neuroblastoma cells via de-regulating the Beclin-1/Bcl-2 complex and inducing apoptosis.
    Authors: Rahman Et al.
    Biochemical Pharmacology  2016;117:97
  26. STAT3 signaling contributes to the high effector activities of interleukin-15-derived dendritic cells.
    Authors: Okada Et al.
    Int J Mol Med  2015;93:461
  27. Hyperphosphate-Induced Myocardial Hypertrophy through the GATA-4/NFAT-3 Signaling Pathway Is Attenuated by ERK Inhibitor Treatment.
    Authors: Liu Et al.
    Biogerontology  2015;5:79
  28. Analysis of the anti-proliferative and the pro-apoptotic efficacy of Syk inhibition in multiple myeloma.
    Authors: Koerber Et al.
    Mol Vis  2015;4:21
  29. MAPK signaling downstream to TLR4 contributes to PacT.-induced peripheral neuropathy.
    Authors: Li Et al.
    Brain Behav.Immun.  2015;49:255
  30. Developmental alterations in centrosome integrity contribute to the post-mitotic state of mammalian cardiomyocytes.
    Authors: Zebrowski Et al.
    Mol Vis  2015;4
  31. MFG-E8 inhibits neutrophil migration through αvβ3-integrin-dependent MAP kinase activation.
    Authors: Aziz Et al.
    Elife  2015;36:18
  32. Regulation of the hyperosmotic induction of aquaporin 5 and VEGF in retinal pigment epithelial cells: involvement of NFAT5.
    Authors: Hollborn Et al.
    Oncotarget  2015;21:360
  33. Gene expression regulation in retinal pigment epithelial cells induced by viral RNA and viral/bacterial DNA.
    Authors: Brosig Et al.
    Immunol Cell Biol  2015;21:1000
  34. P2X7 Receptor Regulates Internalization of Antimicrobial Peptide LL-37 by Human Macrophages That Promotes Intracellular Pathogen Clearance.
    Authors: Tang Et al.
    J Immunol  2015;195:1191
  35. Macrophage migration inhibitory factor-CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors.
    Authors: Lourenco Et al.
    J Immunol  2015;194:3463
  36. Nijmegen breakage syndrome fibroblasts expressing the C-terminal truncated NBN(p70) protein undergo p38/MK2-dependent premature senescence.
    Authors: Davis Et al.
    Exp Hematol Oncol  2015;16:43
  37. JNK2 controls fragmentation of the Golgi complex and the G2/M transition through phosphorylation of GRASP65.
    Authors: Cervigni Et al.
    J Cell Sci  2015;128:2249
  38. 1α,25-Dihydroxyvitamin D3 Induces Neutrophil Apoptosis through the p38 MAPK Signaling Pathway in Chronic Obstructive Pulmonary Disease Patients.
    Authors: Yang Et al.
    PLoS One  2015;10:e0120515
  39. Differential effects of the Toll-like receptor 2 agonists, PGN and Pam3CSK4 on anti-IgE induced human mast cell activation.
    Authors: Yu Et al.
    PLoS One  2014;9:e112989
  40. Distinct signalling pathways of murine histamine H1- and H4-receptors expressed at comparable levels in HEK293 cells.
    Authors: Beermann Et al.
    Respir Res  2014;9:e107481
  41. Angiogenic growth factors augment K-Cl cotransporter expression in erythroid cells via hypoxia-inducible factor-1α.
    Authors: Gonsalves Et al.
    Am J Hematol  2014;89:273
  42. AMP-activated protein kinase determines apoptotic sensitivity of cancer cells to ginsenoside-Rh2.
    Authors: Kim Et al.
    J Ginseng Res  2014;38:16
  43. Central SDF-1/CXCL12 expression and its cardiovascular and sympathetic effects: the role of angiotensin II, TNF-α, and MAP kinase signaling.
    Authors: Wei Et al.
    Am J Physiol Heart Circ Physiol  2014;307:H1643
  44. Activation of pattern recognition receptors in brown adipocytes induces inflammation and suppresses uncoupling protein 1 expression and mitochondrial respiration.
    Authors: Bae Et al.
    Am J Physiol Cell Physiol  2014;306:C918
  45. Cathelicidin LL-37 induces time-resolved release of LTB4 and TXA2 by human macrophages and triggers eicosanoid generation in vivo.
    Authors: Wan Et al.
    FASEB J  2014;28:3456
  46. Network dynamics determine the autocrine and paracrine signaling functions of TNF.
    Authors: Caldwell Et al.
    Genes Dev  2014;28:2120
  47. ERK/MAPK regulates ERRγ expression, transcriptional activity and receptor-mediated tamox. resistance in ER+ breast cancer.
    Authors: Heckler Et al.
    FEBS J  2014;281:2431
  48. Decrease of PKB/Akt Phosphorylation is Partially Mediated by SAPK/JNK Activation in Serum-free L6 Myoblasts Starved with Low Glucose.
    Authors: Kim Et al.
    J Phys Ther Sci  2014;26:1757
  49. p38 MAPK signaling underlies a cell-autonomous loss of stem cell self-renewal in skeletal muscle of aged mice.
    Authors: Bernet Et al.
    Korean J Physiol Pharmacol  2014;20:265
  50. δ-Opioid receptors up-regulate excitatory amino acid transporters in mouse astrocytes.
    Authors: Liang Et al.
    Exp Eye Res  2014;171:5417
  51. Transcriptional regulation of an Ins-sensitizing adipokine adipolin/CTRP12 in adipocytes by Krüppel-like factor 15.
    Authors: Enomoto Et al.
    Mol Med  2013;8:e83183
  52. Fibroblast growth factor-4 enhances proliferation of mouse embryonic stem cells via activation of c-Jun signaling.
    Authors: Kook Et al.
    PLoS One  2013;8:e71641
  53. A novel model of dormancy for bone metastatic breast cancer cells.
    Authors: Marlow Et al.
    Cancer Res  2013;73:6886
  54. Impaired glutamate recycling and GluN2B-mediated neuronal calcium overload in mice lacking TGF-β1 in the CNS.
    Authors: Koeglsperger Et al.
    Glia  2013;61:985
  55. Effect of thiorid. on erythrocytes.
    Authors: Lang Et al.
    Toxins (Basel)  2013;5:1918
  56. EI24 regulates epithelial-to-mesenchymal transition and tumor progression by suppressing TRAF2-mediated NF-κB activity.
    Authors: Choi Et al.
    PLoS One  2013;4:2383
  57. Epigallocatechin gallate (EGCG) stimulates autophagy in vascular endothelial cells: a potential role for reducing lipid accumulation.
    Authors: Kim Et al.
    J Biol Chem  2013;288:22693
  58. A Taiwanese Propolis Derivative Induces Apoptosis through Inducing Endoplasmic Reticular Stress and Activating Transcription Factor-3 in Human Hepatoma Cells.
    Authors: Suk Et al.
    Evid Based Complement Alternat Med  2013;2013:658370
  59. p38α senses environmental stress to control innate immune responses via mechanistic target of rapamycin.
    Authors: Katholnig Et al.
    J Immunol  2013;190:1519
  60. An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis during coxsackievirus B3 infection.
    Authors: Jensen Et al.
    J Neurosci  2013;13:67
  61. Inhibition of a novel specific neuroglial integrin signaling pathway increases STAT3-mediated CNTF expression.
    Authors: Keasey Et al.
    Cell Commun Signal  2013;11:35
  62. Gremlin utilizes canonical and non-canonical TGFβ signaling to induce lysyl oxidase (LOX) genes in human trabecular meshwork cells.
    Authors: Sethi Et al.
    J Neuroinflammation  2013;113:117
  63. Using the one-lung method to link p38 to pro-inflammatory gene expression during overventilation in C57BL/6 and BALB/c mice.
    Authors: Siegl
    PLoS One  2012;7:e41464
  64. Distinct regulatory functions of calpain 1 and 2 during neural stem cell self-renewal and differentiation.
    Authors: Santos Et al.
    PLoS One  2012;7:e33468
  65. TLR signaling paralyzes monocyte chemotaxis through synergized effects of p38 MAPK and global Rap-1 activation.
    Authors: Yi Et al.
    PLoS One  2012;7:e30404
  66. Rit GTPase regulates a p38 MAPK-dependent neuronal survival pathway.
    Authors: Cai Et al.
    J Cell Mol Med  2012;531:125
  67. Fibroblast growth factor-23 abolishes 1,25-dihydroxyvitamin D3-enhanced duodenal calcium transport in male mice.
    Authors: Khuituan Et al.
    Am J Physiol Endocrinol Metab  2012;302:E903
  68. TAK-1/p38/nNFκB signaling inhibits myoblast differentiation by increasing levels of AVNA.
    Authors: Trendelenburg Et al.
    Skelet Muscle  2012;2:3
  69. Inhibition of α-SMA by the ectodomain of FGFR2c attenuates lung fibrosis.
    Authors: Ju Et al.
    Diabetologia  2012;18:992
  70. Cyclic AMP increases COX-2 expression via mitogen-activated kinase in human myometrial cells.
    Authors: Chen Et al.
    PLoS One  2012;16:1447
  71. Inflammatory muscle pain is dependent on the activation of kinin B1 and B2 receptors and intracellular kinase pathways.
    Authors: Meotti Et al.
    Br J Pharmacol  2012;166:1127
  72. The neuropeptide PACAP38 induces dendritic spine remodeling through ADAM10-N-cadherin signaling pathway.
    Authors: Gardoni Et al.
    J Cell Sci  2012;125:1401
  73. Brain microvascular pericytes are immunoactive in culture: cytokine, chemokine, nitric oxide, and LRP-1 expression in response to lipopolysaccharide.
    Authors: Kovac Et al.
    J Biol Chem  2011;8:139
  74. A screen for regulators of survival of motor neuron protein levels.
    Authors: Makhortova Et al.
    Nat Chem Biol  2011;7:544
  75. Mitochondrial localized STAT3 is involved in NGF induced neurite outgrowth.
    Authors: Zhou and Too
    Nat Med  2011;6:e21680
  76. Role of TGFβ/Smad signaling in gremlin induction of human trabecular meshwork extracellular matrix proteins.
    Authors: Sethi Et al.
    PLoS One  2011;52:5251
  77. Induction of steroid sulfatase expression by tumor necrosis factor-α through phosphatidylinositol 3-kinase/Akt signaling pathway in PC-3 human prostate cancer cells.
    Authors: Suh Et al.
    Exp Mol Med  2011;43:646
  78. Reversible differentiation of myofibroblasts by MyoD.
    Authors: Hecker Et al.
    J Neurosci  2011;317:1914
  79. Real-time imaging reveals that P2Y2 and P2Y12 receptor agonists are not chemoattractants and macrophage chemotaxis to complement C5a is phosphatidylinositol 3-kinase (PI3K)- and p38 mitogen-activated protein kinase (MAPK)-independent.
    Authors: Isfort Et al.
    J Biol Chem  2011;286:44776
  80. MUC5AC expression through bidirectional communication of Notch and epidermal growth factor receptor pathways.
    Authors: Kang Et al.
    J Immunol  2011;187:222
  81. Thrombin-induced Migration and Matrix Metalloproteinase-9 Expression Are Regulated by MAPK and PI3K Pathways in C6 Glioma Cells.
    Authors: Kim Et al.
    Mol Cancer  2011;15:211
  82. Carnosic acid induces the NAD(P)H: quinone oxidoreductase 1 expression in rat clone 9 cells through the p38/nuclear factor erythroid-2 related factor 2 pathway.
    Authors: Tsai Et al.
    J Nutr  2011;141:2119
  83. TWEAK is a positive regulator of cardiomyocyte proliferation.
    Authors: Novoyatleva Et al.
    Toxins (Basel)  2010;85:681
  84. Redox Factor-1 Inhibits Cyclooxygenase-2 Expression via Inhibiting of p38 MAPK in the A549 Cells.
    Authors: Yoo Et al.
    Korean J Physiol Pharmacol  2010;14:139
  85. Cigarette smoke and lipopolysaccharide induce a proliferative airway smooth muscle phenotype.
    Authors: Pera Et al.
    Exp Cell Res  2010;11:48
  86. miR-155 and its star-form partner miR-155* cooperatively regulate type I IF. production by human plasmacytoid dendritic cells.
    Authors: Zhou Et al.
    Blood  2010;116:5885
  87. PLZF is a regulator of homeostatic and cytokine-induced myeloid development.
    Authors: Doulatov Et al.
    Genes Dev  2009;23:2076
  88. Ethanol augments RANTES/CCL5 expression in rat liver sinusoidal endothelial cells and human endothelial cells via activation of NF-kappa B, HIF-1 alpha, and AP-1.
    Authors: Yeligar Et al.
    J Immunol  2009;183:5964
  89. Transcriptional regulation of monocyte chemotactic protein-1 release by endothelin-1 in human airway smooth muscle cells involves NF-kappaB and AP-1.
    Authors: Sutcliffe Et al.
    Br J Pharmacol  2009;157:436
  90. The expression of the beta-defensins hBD-2 and hBD-3 is differentially regulated by NF-kappaB and MAPK/AP-1 pathways in an in vitro model of Candida esophagitis.
    Authors: Steubesand Et al.
    BMC Immunol  2009;10:36
  91. Assessing the role of stress signalling via p38 MAP kinase in the premature senescence of ataxia telangiectasia and Werner syndrome fibroblasts.
    Authors: Davis and Kipling
    Biogerontology  2009;10:253
  92. Mitogen-activated protein kinases mediate upregulation of hypothalamic angiotensin II type 1 receptors in heart failure rats.
    Authors: Wei Et al.
    Hypertension  2008;52:679
  93. Reactive oxygen species stabilize hypoxia-inducible factor-1 alpha protein and stimulate transcriptional activity via AMP-activated protein kinase in DU145 human prostate cancer cells.
    Authors: Jung Et al.
    Carcinogenesis  2008;29:713
  94. Kinase networks integrate profiles of N-MthD.-aspartate receptor-mediated gene expression in hippocampus.
    Authors: Coba Et al.
    J Biol Chem  2008;283:34101
  95. Induction of human beta-defensin 2 by the probiotic Escherichia coli Nissle 1917 is mediated through flagellin.
    Authors: Schlee Et al.
    Infect Immun  2007;75:2399
  96. Delayed and persistent ERK1/2 activation is required for 4-hydroxytamoxifen-induced cell death.
    Authors: Zhou Et al.
    Invest Ophthalmol Vis Sci  2007;72:765
  97. Requirement of extracellular signal-regulated kinase/mitogen-activated protein kinase for long-term potentiation in adult mouse anterior cingulate cortex.
    Authors: Toyoda Et al.
    Mol Pain  2007;3:36
  98. Somatic excision demonstrates that c-Jun induces cellular migration and invasion through induction of stem cell factor.
    Authors: Katiyar Et al.
    Mol Cell Biol  2007;27:1356
  99. PacT.-induced nuclear translocation of FOXO3a in breast cancer cells is mediated by c-Jun NH2-terminal kinase and Akt.
    Authors: Sunters Et al.
    Cancer Res  2006;66:212
  100. Role of glycogen synthase kinase 3 β (GSK3β) in mediating the cytotoxic effects of the histone deacetylase inhibitor trichostatin A (TSA) in MCF-7 breast cancer cells.
    Authors: Alao Et al.
    Steroids  2006;5:40
  101. The role of protein kinase B/Akt in Ins-induced inactivation of phosphorylase in rat hepatocytes.
    Authors: Aiston Et al.
    Cardiovasc Res  2006;49:174
  102. Regulation of nuclear factor κB in the hippocampus by group I metabotropic glutamate receptors.
    Authors: O'Riordan Et al.
    Cell Host Microbe  2006;26:4870
  103. Direct phosphorylation and regulation of poly(ADP-ribose) polymerase-1 by extracellular signal-regulated kinases 1/2.
    Authors: Kauppinen Et al.
    Proc Natl Acad Sci U S A  2006;103:7136
  104. beta-Amyloid peptides impair PKC-dependent functions of metabotropic glutamate receptors in prefrontal cortical neurons.
    Authors: Tyszkiewicz and Yan
    J Neurophysiol  2005;93:3102
  105. Glucose deprivation increases mRNA stability of vascular endothelial growth factor through activation of AMP-activated protein kinase in DU145 prostate carcinoma.
    Authors: Yun Et al.
    Nat Commun  2005;280:9963
  106. Extracellular signal-regulated protein kinase activation is required for metabotropic glutamate receptor-dependent long-term depression in hippocampal area CA1.
    Authors: Gallagher Et al.
    Neurosci Lett  2004;24:4859


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Works for cancer cell lines
By Anonymous on 07/20/2021
Species: Human

Product was dissolved in DMSO, and was used at a concentration of 10 µM in a cancer cell line.

Worked for migration assay
By Anonymous on 07/01/2021
Species: Human

Used MAPK inhibitors at 10 μM: UO126: MEK1/MEK2 inhibitor, SB203580: p38 inhibitor, ZM336372: c-RAF inhibitor.

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