SB 203580

Tocris Bioscience | Catalog # 1202

Selective inhibitor of p38 MAPK
Tocris Bioscience

Key Product Details

Description

Selective inhibitor of p38 MAPK
View all p38 Inhibitors »

Product Description

SB 203580 is a selective inhibitor of p38 MAPK (IC50 values are 50 and 500 nM for SAPK2a/p38 and SAPK2b/p38β2 respectively). Displays 100-500-fold selectivity over LCK, GSK-3β and PKBα. Shown to inhibit IL-2-induced T cell proliferation, cyclooxygenase-1 and -2, and thromboxane synthase. Enhances clonal growth of skin epithelial progenitor cells; stimulates neural stem cell (NSC) proliferation. SB 203580 is an essential component of medium for maintaining stem cells in naive pluripotent state and can be used to promote expansion of HSCs ex vivo.

Water-soluble Salt also available.

Product Specifications for SB 203580

Molecular Weight

377.44

Formula

C21H16FN3OS

Storage

Store at +4°C

Purity

≥98% (HPLC)

Chemical Name

4-[5-(4-Fluorophenyl)-2-[4-(methylsulfonyl)phenyl]-1H-imidazol-4-yl]pyridine

CAS Number

152121-47-6

PubChem ID

176155

InChI Key

CDMGBJANTYXAIV-UHFFFAOYSA-N

SMILES

CS(=O)C1=CC=C(C=C1)C1=NC(=C(N1)C1=CC=NC=C1)C1=CC=C(F)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 9.44 25

Preparing Stock Solutions for SB 203580

The following data is based on the product molecular weight 377.44.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
0.25 mM 10.60 mL 52.99 mL 105.98 mL
1.25 mM 2.12 mL 10.60 mL 21.20 mL
2.5 mM 1.06 mL 5.30 mL 10.60 mL
12.5 mM 0.21 mL 1.06 mL 2.12 mL

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Background References

References are publications that support the biological activity of the product. See our Citations tab to view 715 publications citing the usage of this product.

Product Documents for SB 203580

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SB 203580

For research use only

Customer Reviews for SB 203580 (6)

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  • Combinatorial treatments
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 10/18/2022
    Combinatorial treatments of the p38 MAPK inhibitor with chemotherapeutics led to synergistic apoptotic phenotype in vitro. The product is highly recommended.
  • Works for cancer cell lines
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 07/20/2021
    Product was dissolved in DMSO, and was used at a concentration of 10 µM in a cancer cell line.
  • Worked for migration assay
    Name: Anonymous
    Species: Human
    Verified Customer | Posted 07/01/2021
    Used MAPK inhibitors at 10 μM: UO126: MEK1/MEK2 inhibitor, SB203580: p38 inhibitor, ZM336372: c-RAF inhibitor.
  • The role of p38 MAPK signaling in genotoxics-induced stress.
    Name: MARINA KOUTSIOUMPA
    Species: Human
    Assay Type: In Vitro
    Verified Customer | Posted 06/10/2021
    SB 203580 was dissolved in DMSO and used at a final concentration of 1, 3 or 10 µM in human cancer cell lines, followed by treatment with genotoxics.
  • Used for Migration assays
    Name: Anonymous
    Species: Human
    Assay Type: In Vitro
    Cell Line/Tissue: A549 or MDAMB231
    Verified Customer | Posted 07/01/2020
    We ordered this product, combining with another inhibitor, to confirm the migration.To confirm that tested product induces migration, we also screen all MAPK inhibitors and MIF receptor antagonists
    All MAPK inhibitors (ZM336372: c-RAF inhibitor; UO126: MEK1/MEK2 inhibitor, SB203580: p38 inhibitor) were used at 10 μM
    SB 203580 1202
  • The role of p38 MAPK signaling in LPA induced pro-inflammatory phenotype in microglia cells
    Name: Joanna Plastira
    Species: Mouse
    Assay Type: In Vitro
    Cell Line/Tissue: Primary murine microglia cells
    Verified Customer | Posted 06/08/2019
    The role of MAPK pathways (JNK, p38 and ERK) was studied in the LPA induced pro-inflammatory phenotype in microglia. In this case cells were incubated for the indicated time points with LPA (1µM) in the presence or absence of SB239063 (10µM) or SB203580 (10µM). A series of assays were performed. p38 MAPK inhibition totally decreased the expression of 4 pro-inflammatory transcription factors (here presented: pp65). Both inhibitors worked nicely and the results were consistent.
    SB 203580 1202

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