SCAP Antibody (HL2796)

Novus Biologicals | Catalog # NBP3-48733

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2796
View all SCAP Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human SCAP.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SCAP Antibody (HL2796)

SCAP Antibody (HL2796)

Immunohistochemistry-Paraffin: SCAP Antibody (HL2796) [NBP3-48733] -

SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SCAP stained by SCAP antibody [HL2796] (NBP3-48733) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SCAP Antibody (HL2796)

Western Blot: SCAP Antibody (HL2796) [NBP3-48733] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [HL2796] (NBP3-48733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SCAP Antibody (HL2796)

Immunohistochemistry-Paraffin: SCAP Antibody (HL2796) [NBP3-48733] -

SCAP antibody [HL2796] detects SCAP protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
SCAP stained by SCAP antibody [HL2796] (NBP3-48733) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SCAP Antibody (HL2796)

Western Blot: SCAP Antibody (HL2796) [NBP3-48733] -

Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SCAP antibody [HL2796] (NBP3-48733) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SCAP Antibody (HL2796)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SCAP

Sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) is a molecular chaperone that plays a role in the biosynthesis of cholesterol and triglycerides (1). SCAP is a membrane protein that helps shuttle SREBP from the endoplasmic reticulum (ER) via coat protein II (COPII) vesicles to the golgi where SREBP is cleaved via protease S1P and S2P, becomes activated, and is translocated to the nucleus for transcription (1-3). The SCAP protein has a theoretical molecular weight of 140 kDa and is comprised of 1276 amino acids with an N-terminal domain containing 8 transmembrane alpha helices, where transmembrane 2-6 consist of a sterol-sensing domain, and a C-terminal domain with WD40 motif repeats (1,3). The SCAP-SREBP association plays a pivotal role in regulating lipid metabolism and lipogenesis (1,4). SREBP activation via SCAP is associated with accumulation of triglycerides and cholesterol leading to metabolic disorders including diabetes, atherosclerosis, and nonalcoholic fatty liver disease (NAFLD) (1,4). Animal models have revealed that inhibition or blocking of SCAP using antagonists may be a potential therapeutic target for hyperlipidemia and hypertriglyceridemia (1,4).

References

1. Lee, S. H., Lee, J. H., & Im, S. S. (2020). The cellular function of SCAP in metabolic signaling. Experimental & molecular medicine, 52(5), 724-729. https://doi.org/10.1038/s12276-020-0430-0

2. Cheng, X., Li, J., & Guo, D. (2018). SCAP/SREBPs are Central Players in Lipid Metabolism and Novel Metabolic Targets in Cancer Therapy. Current topics in medicinal chemistry, 18(6), 484-493. https://doi.org/10.2174/1568026618666180523104541

3. Brown, M. S., Radhakrishnan, A., & Goldstein, J. L. (2018). Retrospective on Cholesterol Homeostasis: The Central Role of Scap. Annual review of biochemistry, 87, 783-807. https://doi.org/10.1146/annurev-biochem-062917-011852

4. Moon Y. A. (2017). The SCAP/SREBP Pathway: A Mediator of Hepatic Steatosis. Endocrinology and metabolism (Seoul, Korea), 32(1), 6-10. https://doi.org/10.3803/EnM.2017.32.1.6

Alternate Names

KIAA0199, PSEC0227, SREBF chaperone, SREBP cleavage-activating protein, sterol regulatory element binding protein cleavage-activating protein

Gene Symbol

SCAP

Additional SCAP Products

Product Documents for SCAP Antibody (HL2796)

Certificate of Analysis

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Product Specific Notices for SCAP Antibody (HL2796)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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