SCIMP Antibody (NVL-07) - BSA Free

Novus Biologicals | Catalog # NBP2-22349

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # NVL-07

Format

BSA Free
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Product Specifications

Immunogen

Recombinant intracellular part of human SCIMP.

Specificity

The mouse monoclonal antibody NVL-07 recognizes intracellular part of human transmembrane adaptor SCIMP. This protein of 17 kDa predicted Mw migrates as a 22 kDa band on SDS PAGE.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SCIMP Antibody (NVL-07) - BSA Free

Flow Cytometry: SCIMP Antibody (NVL-07) - BSA Free [NBP2-22349]

Flow Cytometry: SCIMP Antibody (NVL-07) - BSA Free [NBP2-22349]

Flow Cytometry: SCIMP Antibody (NVL-07) [NBP2-22349] - Intracellular staining of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.

Applications for SCIMP Antibody (NVL-07) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Purity: > 95% by SDS-PAGE, Flow Cytometry - intracellular staining. This antibody is CyTOF ready.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SCIMP

SCIMP (SLP adaptor and Csk interacting membrane protein), also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It is associated with tetraspanin-enriched microdomains (together with MHC II). There is a close relationship between SCIMP and tyrosinkinase Lyn, which is constitutively bound to it by its SH3 domain. After MHC II-mediated stimulation in the immunological synapse SCIMP becomes phosphorylated at several tyrosine residues and provides docking sites for Grb2 and SLP65 or SLP76 adaptors transducing the signal downstream, as well as for the kinase Csk with modulatory roles.

Alternate Names

C17orf87, chromosome 17 open reading frame 87, DTFT5783, FLJ32580, SLP adapter and CSK-interacting membrane protein, SLP adaptor and CSK interacting membrane protein, SLP65/SLP76, Csk-interacting membrane protein, transmembrane protein C17orf87, UNQ5783

Gene Symbol

SCIMP

UniProt

Q6UWF3 (Human)

Additional SCIMP Products

Product Documents for SCIMP Antibody (NVL-07) - BSA Free

Certificate of Analysis

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Product Specific Notices for SCIMP Antibody (NVL-07) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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