SCN3B Antibody (S396-29) - BSA Free

Novus Biologicals | Catalog # NBP2-59318

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # S396-29

Format

BSA Free
View all SCN3B Primary Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 1-215 (full-length) of rat NavBeta3

Localization

Membrane

Specificity

Detects 40kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SCN3B Antibody (S396-29) - BSA Free

Western Blot: SCN3B Antibody (S396-29) [NBP2-59318]

Western Blot: SCN3B Antibody (S396-29) [NBP2-59318]

Western Blot: SCN3B Antibody (S396-29) [NBP2-59318] - Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29 (NBP2-59318). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody (NBP2-59318) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.
Immunocytochemistry/ Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318]

Immunocytochemistry/ Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318]

Immunocytochemistry/Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta3 Monoclonal Antibody, Clone S396-29 (NBP2-59318). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav beta3 Monoclonal Antibody (NBP2-59318) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav beta3 Antibody (D) Composite.
Immunocytochemistry/ Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318]

Immunocytochemistry/ Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318]

Immunocytochemistry/Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.

Applications for SCN3B Antibody (S396-29) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
This SCN3B Antibody (S396-29) has been validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: separated by Size

Reviewed Applications

Read 1 review rated 4 using NBP2-59318 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: SCN3B

Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified. (provided by RefSeq)

Alternate Names

HSA243396, KIAA1158, SCNB3, sodium channel subunit beta-3, sodium channel, voltage-gated, type III, beta, voltage-gated sodium channel beta-3 subunit

Gene Symbol

SCN3B

Additional SCN3B Products

Product Documents for SCN3B Antibody (S396-29) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SCN3B Antibody (S396-29) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SCN3B Antibody (S396-29) - BSA Free

Customer Reviews for SCN3B Antibody (S396-29) - BSA Free (1)

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Customer Images

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  • SCN3B Antibody (S396-29)
    Name: auriane ernault
    Application: Simple Western
    Sample Tested: primary neonatal ventricular cardiomyocytes
    Species: Rat
    Verified Customer | Posted 02/17/2021
    Clear band without noise at 86kDa. Expected molecular weight should be around 30-40kDa. It can be the result of SCN3B dimers.
    Antibody used at a dilution of 1:50. Protein concentration of 0.8ug/uL.
    SCN3B Antibody (S396-29) - BSA Free NBP2-59318
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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