SDHB Antibody (CL0347) - BSA Free

Novus Biologicals | Catalog # NBP2-52920

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0347

Format

BSA Free
View all SDHB Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P21912, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SDHB Antibody (CL0347) - BSA Free

Western Blot: SDHB Antibody (CL0347) [NBP2-52920]

Western Blot: SDHB Antibody (CL0347) [NBP2-52920]

Western Blot: SDHB Antibody (CL0347) [NBP2-52920] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human ventricular cancer shows strong granular cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human breast cancer shows strong granular cytoplasmic immunoreactivity in tumor cells.
Immunohistochemistry: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human colorectal cancer shows strong granular cytoplasmic positivity in tumor cells.
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human kidney shows strong granular positivity in the cytoplasm of renal tubules cells.
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human liver cancer shows strong positivity in tumor cells.
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human rectum shows strong granular cytoplasmic immunoreactivity in glandular cells.
Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920]

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human testis shows strong granular cytoplasmic positivity in seminiferous tubules.

Applications for SDHB Antibody (CL0347) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 1 review rated 3 using NBP2-52920 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SDHB

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Alternate Names

EC 1.3.5.1, FLJ92337, Ip, Iron-sulfur subunit of complex II, PGL4, SDH, SDH1, SDH2, SDHIP, succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Gene Symbol

SDHB

Additional SDHB Products

Product Documents for SDHB Antibody (CL0347) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SDHB Antibody (CL0347) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SDHB Antibody (CL0347) - BSA Free (1)

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  • SDHB Antibody (CL0347)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: glioblastoma cell lysates
    Species: Human
    Verified Customer | Posted 11/04/2023
    lane1, 3: control lane2, 4: drug treated glioblastoma whole cell lysate, 10 ug each marker: 75, 50, 37, 25 kDa
    SDHB Antibody (CL0347) - BSA Free NBP2-52920

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Protocols

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FAQs

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