Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Chicken (100%), Mouse (100%), Orangutan (100%), Rat (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SEC22B Primary Antibodies »

Product Specifications

Immunogen

This antibody maps to a region between residue 100 to 150 of human SEC22 Vesicle Trafficking Protein Homolog B using the numbering given in entry NP_004883.3 (GeneID 9554).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC22B Antibody - BSA Free

Western Blot: SEC22B Antibody [NBP2-37701]

Western Blot: SEC22B Antibody [NBP2-37701]

Western Blot: SEC22B Antibody [NBP2-37701] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Affinity purified rabbit anti-SEC22B antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: SEC22B Antibody [NBP2-37701]

Immunoprecipitation: SEC22B Antibody [NBP2-37701]

Immunoprecipitation: SEC22B Antibody [NBP2-37701] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using RIPA lysis buffer. Antibodies: Affinity purified rabbit anti-SEC22B antibody used for IP at 6 ug per reaction. SEC22B was also immunoprecipitated by rabbit anti-SEC22B antibody. For blotting immunoprecipitated SEC22B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for SEC22B Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:500 - 1:2500
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SEC22B

SEC22B is encoded by this gene is a member of the SEC22 family of vesicle trafficking proteins. It seems to complex with SNARE and it is thought to play a role in the ER-Golgi protein trafficking. This protein has strong similarity to Mus musculus and Cricetulus griseus proteins. There is evidence for use of multiple polyadenylation sites for the transcript.

Alternate Names

ERS24, ERS-24sec22b, SEC22 vesicle trafficking protein homolog B (S. cerevisiae), SEC22 vesicle trafficking protein homolog B (S. cerevisiae) (gene/pseudogene), SEC22 vesicle trafficking protein-like 1, SEC22 vesicle trafficking protein-like 1 (S. cerevisiae), SEC22 vesicle-trafficking protein homolog B, SEC22 vesicle-trafficking protein-like 1, SEC22, vesicle trafficking protein (S. cerevisiae)-like 1, SEC22L1ER-Golgi SNARE of 24 kDa, vesicle-trafficking protein SEC22b

Gene Symbol

SEC22B

UniProt

NP_004883.3 (Human)

Additional SEC22B Products

Product Documents for SEC22B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SEC22B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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