SEC23A Antibody

Novus Biologicals | Catalog # NBP2-34842

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SEC23A Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues M(1) T T Y L E F I Q Q N E E R D G V R (18) C of rat COPII.

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 6452637).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC23A Antibody

Western Blot: SEC23A Antibody [NBP2-34842]

Western Blot: SEC23A Antibody [NBP2-34842]

Western Blot: SEC23A Antibody [NBP2-34842] - Analysis was performed whole cell extracts and tissue extracts of HeLa (Lane 1), PC-12 (Lane 2), NIH/3T3 (Lane 3), Mouse Liver (Lane 4) and Rat Liver (Lane 5).
Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).
Flow Cytometry: SEC23A Antibody [NBP2-34842]

Flow Cytometry: SEC23A Antibody [NBP2-34842]

Flow Cytometry: SEC23A Antibody [NBP2-34842] - Analysis of COP II was done on PC-12 cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton (R) X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with COP II Rabbit Polyclonal Antibody (PA1-069A, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.
Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of PC12 cells (right) compared to a negative control without primary antibody (left).
Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/ Immunofluorescence: SEC23A Antibody [NBP2-34842]

Immunocytochemistry/Immunofluorescence: SEC23A Antibody [NBP2-34842] - Analysis of COP II (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).

Applications for SEC23A Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:200

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

2 ug/ml
Application Notes
Useful in WB. WB: Detects an approx. 85 kDa protein representing COPII from PC-12 cell extract. May be useful in ICC and IP as reported in scientific literature.

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SEC23A

Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two Coatomer-Protein mechanisms (COPI and COPII) and although they have mechanistic parallels, they are molecularly distinct. The COPI coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COPI) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COPI, and ARF. COPI has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COPII facilitates anterograde transport between these subcellular organelles. COPII has been shown to be independently and selectively recruited to the ER relative to COPI subunits.

Alternate Names

CLSD, MGC26267, protein transport protein Sec23A, Sec23 (S. cerevisiae) homolog A, Sec23 homolog A (S. cerevisiae), SEC23-related protein A

Entrez Gene IDs

26958 (Human); 54160 (Mouse); 58847 (Rat)

Gene Symbol

SEC23A

UniProt

B5DFC3 (Rat)

Additional SEC23A Products

Product Documents for SEC23A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SEC23A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SEC23A Antibody

Customer Reviews for SEC23A Antibody

There are currently no reviews for this product. Be the first to review SEC23A Antibody and earn rewards!

Have you used SEC23A Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies