SEC61A Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-94505

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all SEC61A Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 310-420 of human SEC61A1 (NP_037468.1). ARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPESFGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA

Specificity

This antibody made against SEC61A1 but the immunogen shows high sequence identity with human SEC61A2. Reactivity with SEC61A2 has not been determined experimentally.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SEC61A Antibody - Azide and BSA Free

Western Blot: SEC61A AntibodyAzide and BSA Free [NBP2-94505]

Western Blot: SEC61A AntibodyAzide and BSA Free [NBP2-94505]

Western Blot: SEC61A Antibody [NBP2-94505] - Analysis of extracts of A-549 cells, using SEC61A.Exposure time: 90s.
SEC61A Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] -

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] - Immunofluorescence analysis of HeLa cells using SEC61A Rabbit pAb (A11614) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
SEC61A Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] -

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] - Immunofluorescence analysis of PC-12 cells using SEC61A Rabbit pAb (A11614) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
SEC61A Antibody - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] -

Immunocytochemistry/ Immunofluorescence: SEC61A Antibody - Azide and BSA Free [NBP2-94505] - Immunofluorescence analysis of NIH/3T3 cells using SEC61A Rabbit pAb (A11614) at dilution of 1:300 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for SEC61A Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SEC61A

SEC61A belongs to the SECY/SEC61 alpha family. It has similarity to a mouse protein which suggests a role in the insertion of secretory and membrane polypeptides into the endoplasmic reticulum.The SEC61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. SEC61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. SEC61 alpha is a polytopic protein that has ten membrane-spanning loops. SEC61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.

Alternate Names

HSEC61, protein transport protein SEC61 alpha subunit, protein transport protein Sec61 subunit alpha, protein transport protein Sec61 subunit alpha isoform 1, SEC61, Sec61 alpha 1 subunit (S. cerevisiae), Sec61 alpha-1, sec61 homolog, SEC61A

Gene Symbol

SEC61A1

Additional SEC61A Products

Product Documents for SEC61A Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SEC61A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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