Semaphorin 3E Antibody
Novus Biologicals | Catalog # NB100-55417
![Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417]](https://resources.rndsystems.com/images/products/Semaphorin-3E-Antibody-Immunohistochemistry-Paraffin-NB100-55417-img0003.jpg "Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417]")
Loading...
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Peptide with sequence C-NGGRYGTTKDYPDD corresponding to internal region according to NP_036563.1, NP_001171600.1.
Epitope
NGGRYGTTKDYPDD
Specificity
This antibody is expected to recognise both reported isoforms (NP_036563.1; NP_001171600.1).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Semaphorin 3E Antibody
Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417]
Immunohistochemistry-Paraffin: Semaphorin 3E Antibody [NB100-55417] - Staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.Applications for Semaphorin 3E Antibody
Application
Recommended Usage
Immunohistochemistry
4 - 6 ug/ml
Immunohistochemistry-Paraffin
1:200
Peptide ELISA
Detection limit 1:128000
Application Notes
WB: Preliminary experiments gave an approx. 35 kDa band in human prostate lysates after 0.03 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 89.2 kDa band according to NP_036563.1. The 35 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human prostate shows membranous staining of secretory cells in the gland.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Semaphorin 3E
Alternate Names
Sema3E, SEMAH
Gene Symbol
SEMA3E
UniProt
Additional Semaphorin 3E Products
Product Documents for Semaphorin 3E Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Semaphorin 3E Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Semaphorin 3E Antibody
Powered by Bioz
Customer Reviews for Semaphorin 3E Antibody
There are currently no reviews for this product. Be the first to review Semaphorin 3E Antibody and earn rewards!
Have you used Semaphorin 3E Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...