Semaphorin 4B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19299

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Semaphorin 4B Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Semaphorin 4B. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (87%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Semaphorin 4B Antibody - BSA Free

Western Blot: Semaphorin 4B Antibody [NBP2-19299]

Western Blot: Semaphorin 4B Antibody [NBP2-19299]

Western Blot: Semaphorin 4B Antibody [NBP2-19299] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - Mouse intestine. SEMA4B antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - U373 xenograft, using SEMA4B antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - Colon carcinoma. SEMA4B antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299]

Immunohistochemistry-Paraffin: Semaphorin 4B Antibody [NBP2-19299] - Mouse intestine. SEMA4B antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Semaphorin 4B Antibody

Western Blot: Semaphorin 4B Antibody [NBP2-19299] -

Western Blot: Semaphorin 4B Antibody [NBP2-19299] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Semaphorin 4B antibody (NBP2-19299) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Semaphorin 4B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Semaphorin 4B

Binds to F-actin in a calcium-independent manner. Has no direct effect on actin depolymerization.

Alternate Names

SEMA4B, SEMAC, SemC

Gene Symbol

SEMA4B

Additional Semaphorin 4B Products

Product Documents for Semaphorin 4B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Semaphorin 4B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Proteoglycans

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Protocols

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