Septin-4 Antibody

Novus Biologicals | Catalog # NBP1-52096

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

Peptide with sequence C-ELSKFVKDFSGN corresponding to internal region according to NP_004565.1, NP_536340.1, NP_536341.1, NP_001185642.1.

Epitope

C-ELSKFVKDFSGN

Specificity

This antibody is expected to recognize all reported isoforms (NP_004565.1; NP_536340.1; NP_536341.1, NP_001185642.1). Amino acid numbering in name refers to NP_004565.1 sequence.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Septin-4 Antibody

Western Blot: Septin-4 Antibody [NBP1-52096]

Western Blot: Septin-4 Antibody [NBP1-52096]

Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of 1Human Cerebellum (A) and Mouse Brain (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Flow Cytometry: Septin-4 Antibody [NBP1-52096]

Flow Cytometry: Septin-4 Antibody [NBP1-52096]

Flow Cytometry: Septin-4 Antibody [NBP1-52096] - Analysis of paraformaldehyde fixed Jurkat cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: Septin-4 Antibody [NBP1-52096]

Western Blot: Septin-4 Antibody [NBP1-52096]

Western Blot: Septin-4 Antibody [NBP1-52096] - (0.3ug/ml) staining of Jurkat nuclear (A) and LNCaP (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Septin-4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.1-0.3 ug/mL
Application Notes
WB: Approx 55kDa band observed in nuclear lysates of cell lines Jurkat and in LNCaP cell lysates, 50-55kDa in Human Cerebellum lysates, and approx. 50kDa in Mouse Brain lysates (calculated MW of 53.0kDa according to Human NP_536341.1 and 49.4kDa according to Mouse NP_001271323.1).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Septin-4

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. The protein encoded by this gene is thought to be part of a complex involved in cytokinesis. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Alternate Names

Apoptosis-related protein in the TGF-beta signaling pathway, ARTSPeanut-like protein 2, BRADEION, Bradeion beta, Brain protein H5, CE5B3, Cell division control-related protein 2, Cerebral protein 7, H5, hCDCREL-2SEP4, hucep-7, MART, peanut-like 2, peanut-like 2 (Drosophila), PNUTL2CE5B3 beta, septin 4, septin-4, septin-M

Entrez Gene IDs

5414 (Human); 18952 (Mouse)

Gene Symbol

SEPTIN4

UniProt

NP_004565.1 (Human)

Additional Septin-4 Products

Product Documents for Septin-4 Antibody

Certificate of Analysis

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Product Specific Notices for Septin-4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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