Serine racemase Antibody (12B2) - BSA Free

Novus Biologicals | Catalog # NBP3-26302

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 12B2 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human Serine racemase [UniProt Q9GZT4]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Serine racemase Antibody (12B2) - BSA Free

Serine racemase Antibody (12B2)

Western Blot: Serine racemase Antibody (12B2) [NBP3-26302] -

Western Blot: Serine racemase Antibody (12B2) [NBP3-26302] - Positive Western Blot detected in: A549 whole cell lysate, U87 whole cell lysate.
All lanes: Serine racemase Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Serine racemase Antibody (12B2)

Flow Cytometry: Serine racemase Antibody (12B2) [NBP3-26302] -

Flow Cytometry: Serine racemase Antibody (12B2) [NBP3-26302] - Overlay Peak curve showing HepG2 cells stained with Serine racemase Antibody (12B2) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.

Applications for Serine racemase Antibody (12B2) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:200

Western Blot

1:500-1:2000

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Serine racemase

Serine racemase catalyzes the synthesis of phosphoribosylpyrophosphate (PRPP) that is essential for nucleotide synthesis

Alternate Names

D-serine ammonia-lyase, D-serine dehydratase, EC 4.3.1.17, EC 4.3.1.18, EC 5.1.1.18, ILV1, ISO1, L-serine ammonia-lyase, L-serine dehydratase, serine racemase

Gene Symbol

SRR

Additional Serine racemase Products

Product Documents for Serine racemase Antibody (12B2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Serine racemase Antibody (12B2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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