Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Immunoprecipitation

Cited:

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Format

BSA Free
View all Serpin A1/alpha 1-Antitrypsin Primary Antibodies »

Product Specifications

Immunogen

Serpin A1/alpha 1-Antitrypsin [Human Plasma] (Uniprot: P01009)

Reactivity Notes

Cross reactivity against Serpin A1/alpha 1-Antitrypsin from other sources is unknown.

Localization

Secreted

Specificity

Cross reactivity against Serpin A1/alpha 1-Antitrypsin from other sources is unknown.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified a1-Anti-Trypsin [Human Plasma]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Analysis using HRP conjugate of NB600-602. Lane 1: reduced Alpha-1anti-Trypsin. Lane 2: none. Load: 1ug per lane. Primary antibody: Alpha-1-Anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 1.5 hr at RT. Block: incubated with blocking buffer for Alpha-1-AntiTrypsin. Other band(s): none.
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Albumin depleted human serum. Lane 2: none. Load: 10 ug per lane. Primary antibody: none. Secondary antibody: Peroxidase goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 52 kDa, 52 kDa for Alpha-1-Anti-Trypsin. Other band(s): none
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: incubated with blocking buffer for alpha-1 Anti-Trypsin. Other band(s): none
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Primary antibody: Each primary antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: at 30 min RT
Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602]

Western Blot: Serpin A1/alpha 1-Antitrypsin Antibody [NB600-602] - Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4C. Secondary antibody: Rabbit IgG (H&L) Antibody DyLight 649 Conjugated (green), Goat IgG (H&L) Antibody DyLight 549 Conjugated (red), Mouse IgG (H&L) Antibody DyLight 488 Conjugated (blue)secondary antibody at 1:10,000 for 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4C

Applications for Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunoprecipitation

1:10 - 1:500

Western Blot

1:2000-1:10000
Application Notes
This product has been tested by ELISA and western blot. This product has been assayed against 1.0 ug of Alpha-1-Anti-trypsin [Human Plasma] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin A1/alpha 1-Antitrypsin

Alpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.

Alternate Names

A1AT, alpha 1-Antitrypsin, alpha 1-Proteinase Inhibitor

Entrez Gene IDs

5265 (Human)

Gene Symbol

SERPINA1

UniProt

P01009

Additional Serpin A1/alpha 1-Antitrypsin Products

Product Documents for Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Serpin A1/alpha 1-Antitrypsin Antibody - BSA Free

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