Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20108

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-42B-10

Format

Azide and BSA Free
View all Serpin A6 Primary Antibodies »

Product Specifications

Immunogen

Peptide "AQLLQGLGFNLTER" derived from the Corticosteroid-binding globulin SERPINA6, conjugated to KLH.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images

Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free [NBP3-20108] -

Immunocytochemistry/Immunofluorescence: Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free [NBP3-20108] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells stained with the chimeric rabbit IgG version of SAIC-42B-10 (NBP3-20108) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20108, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free

Flow Cytometry: Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free [NBP3-20108] -

Flow Cytometry: Serpin A6/Cortisol Binding Globulin Antibody (SAIC-42B-10) - Azide and BSA Free [NBP3-20108] - Paraformaldehyde fixed HepG2 cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-42B-10 (NBP3-20108, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Ab can be used in immunoMRM.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Serpin A6

Corticol binding globulin (CBG or transcotin) binds with high affinity to cortisol in plasma. Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

CBG

Gene Symbol

SERPINA6

Additional Serpin A6 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Serine Proteases and Regulators

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Protocols

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