SESN2 Antibody

Novus Biologicals | Catalog # NBP1-44989

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot, ELISA, Dot Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SESN2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide taken within amino acid region 1-80 on SESN2 protein.

Localization

Cytoplasm, Nucleus

Specificity

This antibody is specific to SESN2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SESN2 Antibody

Western Blot: SESN2 Antibody [NBP1-44989]

Western Blot: SESN2 Antibody [NBP1-44989]

Western Blot: SESN2 Antibody [NBP1-44989] - Analysis using the FITC conjugate of NBP1-44989. Detection of SESN2 antibody at 1:500 dilution was probed withSESN2 Positive Control sample. MW of SESN2 is approximately 54.6kDa

Applications for SESN2 Antibody

Application
Recommended Usage

Dot Blot

1:10000

ELISA

1:10000

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

.68 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SESN2

Sestrin 2(SESN2), located at human chromosome 1p35.3, belongs to the sestrin family of proteins. These proteins are cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense (1). This family of proteins is only present in multicellular organisms ranging from nematodes to mammals. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes (2). Peroxiredoxins are the enzymes that metabolize peroxides. Expression of these proteins is regulated by p53, a tumor suppressor protein (3). Recently it was reported that sestrin 2 is not a sulfinic peroxiredoxin reductase (4). Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy (5). Depletion of sestrin 2 in human cancer cells reduced the level of autophagy when autophagy was induced in these cells (5). Similar to sestrin 1, sestrin 2 negatively regulates the activity of mammalian target of rapamycin (mTOR), a positive regulator of cell growth (6). Sestrin 2 may be a promising target in the treatment of chronic obstructive pulmonary disease (COPD). It was reported that mutational inactivation of sestrin 2 induced TGF-Beta signaling and partially reduced the emphysema phenotype in mice model of COPD (7). Sestrin 2 might also be a key target in the treatment of cancer. It was shown to activate AMPK and repress mTORC1 signaling. AMPK is an essential mediator of LKB1 tumor suppressor and it is down-regulated in cancer cells (8). Sestrin 2 is approximately a 57.6kDa protein based on the amino acid predicted MW of 57kDa (480 amino acids).

Alternate Names

DKFZp761M0212, DKFZp761M02121, Hi95, HI95sestrin-2, SES2, SEST2hypoxia induced gene 95, sestrin 2

Entrez Gene IDs

83667 (Human)

Gene Symbol

SESN2

UniProt

P58004 (Human)

Additional SESN2 Products

Product Documents for SESN2 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for SESN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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