SETD7/9 Antibody - BSA Free

Novus Biologicals | Catalog # NB100-56664

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation (ChIP)

Cited:

Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SETD7/9 Primary Antibodies »

Product Specifications

Immunogen

Two synthethic peptides corresponding to amino acids 131-145 (GEVNEDGEMTGEKIA) and 336-352 (GYDHSPPGKSGPEAPEW) of human SETD7 were used as immunogen for this antibody.

Specificity

This is specific for Set7/9 (a histone H3 lysine 4 methyltransferase) and does not recognize PR-Set7 (a histone H4 lysine 20 methyltransferase).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SETD7/9 Antibody - BSA Free (NB100-56664) is a polyclonal antibody validated for use in IHC, WB and ChIP. Anti-SETD7/9 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SETD7/9 Antibody - BSA Free

Western Blot: SETD7/9 Antibody [NB100-56664]

Western Blot: SETD7/9 Antibody [NB100-56664]

Western Blot: SETD7/9 Antibody [NB100-56664] - Whole cell extract from MCF7 cells. Image from verified customer review.
Immunohistochemistry-Paraffin: SETD7/9 Antibody [NB100-56664]

Immunohistochemistry-Paraffin: SETD7/9 Antibody [NB100-56664]

Immunohistochemistry-Paraffin: SETD7/9 Antibody [NB100-56664] - Analysis of human small instestine using SETD7 antibody at 1:100.
Western Blot: SETD7/9 Antibody [NB100-56664]

Western Blot: SETD7/9 Antibody [NB100-56664]

Western Blot: SETD7/9 Antibody [NB100-56664] - Analysis of SETD7 in A) MCF7 and B) HeLa lysate using SETD7 antibody at 1:500.

Applications for SETD7/9 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500-1:1000

Reviewed Applications

Read 1 review rated 5 using NB100-56664 in the following applications:

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETD7/9

Diverse signal transduction pathways impinging on the N-terminal tails of histones lead to a number of post-translational modifications including acetylation, phosphorylation, poly(ADP-ribosylation), ubiquitination and methylation. These modifications play critical roles in regulating chromatin structure and gene expression. Histone methyltransferases (HMTases) selectively methylate evolutionarily conserved arginine or lysine residues, primarily in the N-terminal tails of histones H3 and H4. Set7/9 is a histone specific HMTase that methylates histone H3 lysine 4. Set7/9 transfers methyl groups to lysine 4 of histone H3 in complex with S-adenosyl-L-methionine. Human Set7/9 is a 336 amino acid protein.

Alternate Names

EC 2.1.1.43, H3-K4-HMTase SETD7, histone H3-lysine 4-specific methyltransferase, histone-lysine N-methyltransferase SETD7, KIAA1717SET domain-containing protein 7, KMT7FLJ21193, Lysine N-methyltransferase 7, SET domain containing (lysine methyltransferase) 7, SET7/9Histone H3-K4 methyltransferase SETD7, SET7Set9, SET9

Entrez Gene IDs

73251 (Mouse); 288653 (Rat)

Gene Symbol

SETD7

UniProt

Q8WTS6 (Human)

Additional SETD7/9 Products

Product Documents for SETD7/9 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SETD7/9 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SETD7/9 Antibody - BSA Free

Customer Reviews for SETD7/9 Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
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1 Stars
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Whole cell extract form MCF7 cells
    Species: Human
    Verified Customer | Posted 07/17/2014
    SETD 7/9 in MCF-7 cells
    SETD7/9 Antibody - BSA Free NB100-56664

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Protocols

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FAQs

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