SETD8 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93444

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SETD8 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human SETD8.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SETD8 Antibody - BSA Free (NBP2-93444) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SETD8 Antibody - BSA Free

Western Blot: SETD8 AntibodyBSA Free [NBP2-93444]

Western Blot: SETD8 AntibodyBSA Free [NBP2-93444]

Western Blot: SETD8 Antibody [NBP2-93444] - Western blot analysis of extracts of various cell lines, using (NBP2-93444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Western Blot: SETD8 AntibodyBSA Free [NBP2-93444]

Western Blot: SETD8 AntibodyBSA Free [NBP2-93444]

Western Blot: SETD8 Antibody [NBP2-93444] - Western blot analysis of extracts of various cell lines, using (NBP2-93444) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
SETD8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SETD8 Antibody - BSA Free [NBP2-93444] -

Immunocytochemistry/ Immunofluorescence: SETD8 Antibody - BSA Free [NBP2-93444] - Immunofluorescence analysis of NIH-3T3 cells using KMT5A/SETD8 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
SETD8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SETD8 Antibody - BSA Free [NBP2-93444] -

Immunocytochemistry/ Immunofluorescence: SETD8 Antibody - BSA Free [NBP2-93444] - Immunofluorescence analysis of C6 cells using KMT5A/SETD8 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
SETD8 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SETD8 Antibody - BSA Free [NBP2-93444] -

Immunocytochemistry/ Immunofluorescence: SETD8 Antibody - BSA Free [NBP2-93444] - Immunofluorescence analysis of U-2 OS cells using KMT5A/SETD8 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for SETD8 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SETD8

Histone methyltransferase that specifically monomethylates 'Lys-20' of histone H4. H4 'Lys-20'monomethylation is enriched during mitosis and represents a specific tag for epigenetic transcriptional repression.Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes.Required for cell proliferation, probably by contributing to the maintenance of proper higher order structure of DNAduring mitosis. Involved in chromosome condensation and proper cytokinesis. Nucleosomes are preferred as substratecompared to free histones

Long Name

N-lysine methyltransferase KMT5A

Alternate Names

H4-K20-HMTase KMT5A, KMT5A, PR-Set7, PR/SET07, PRSET7, SET07, SET8

Gene Symbol

KMT5A

Additional SETD8 Products

Product Documents for SETD8 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SETD8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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