SETDB1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-84790

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SETDB1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KLRFLIFFDDGYASYVTQSELYPICRPLKKTWEDIEDISCRDFIEEYVTAYPNRPMVLLKSGQLIKTEWEGTWWKSRVEEVDGSLVRILFLDDKRCEWIYRGSTRLEPMFSMKTSSASALEKKQGQLRTRPNMGAVRSKGPVVQYTQD

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23770855)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SETDB1 Antibody - BSA Free (NBP1-84790) is a polyclonal antibody validated for use in IHC and ICC/IF. Anti-SETDB1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SETDB1 Antibody - BSA Free

SETDB1 Antibody - BSA Free Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Staining of human cervix, uterine shows moderate nuclear positivity in squamous epithelial cells.
SETDB1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody - BSA Free [NBP1-84790]

Immunocytochemistry/ Immunofluorescence: SETDB1 Antibody - BSA Free [NBP1-84790]

Staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane & cytosol.
SETDB1 Antibody - BSA Free Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Staining of human lymph node shows moderate nuclear positivity in non-germinal center cells.
SETDB1 Antibody - BSA Free Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Staining of human colon shows strong nuclear positivity in glandular cells.
SETDB1 Antibody - BSA Free Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Immunohistochemistry-Paraffin: SETDB1 Antibody - BSA Free [NBP1-84790]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.

Applications for SETDB1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SETDB1

The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila Trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 039;Enhancer of zeste 039 proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. [supplied by OMIM]

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Entrez Gene IDs

9869 (Human)

Gene Symbol

SETDB1

UniProt

Q15047 (Human)

Additional SETDB1 Products

Product Documents for SETDB1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SETDB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SETDB1 Antibody - BSA Free

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Protocols

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