SETDB1 Antibody

Novus Biologicals | Catalog # NBP2-20321

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (96%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Block/Neutralize, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), In vitro assay

Cited:

Block/Neutralize, In vitro assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SETDB1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SETDB1. The exact sequence is proprietary.

Reactivity Notes

Rat (89%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

143 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit SETDB1 Antibody (NBP2-20321) is a polyclonal antibody validated for use in IHC, WB, IP and ChIP. Anti-SETDB1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SETDB1 Antibody

SETDB1 Antibody

Western Blot: SETDB1 Antibody [NBP2-20321] -

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SETDB1 Antibody

Western Blot: SETDB1 Antibody [NBP2-20321] -

HeLa whole cell and nuclear extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SETDB1 Antibody

Chromatin Immunoprecipitation: SETDB1 Antibody [NBP2-20321] -

Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-SETDB1 antibody. The precipitated DNA was detected by PCR with primer set targeting to P53P2.
SETDB1 Antibody

Immunoprecipitation: SETDB1 Antibody [NBP2-20321] -

SETDB1 antibody [N1], N-term immunoprecipitates SETDB1 protein in IP experiments.
IP samples: HeLa nuclear extract
A. Control with 4 ug of preimmune Rabbit IgG
B. Immunoprecipitation of SETDB1 protein by 4 ug SETDB1 antibody [N1], N-term (NBP2-20321)
5 % SDS-PAGE
The immunoprecipitated SETDB1 protein was detected by SETDB1 antibody [N1], N-term (NBP2-20321) diluted at 1:1000.
[EasyBlot anti-rabbit IgG was used as a secondary reagent]

Applications for SETDB1 Antibody

Application
Recommended Usage

Block/Neutralize

Reported in scientific literature (PMID: 24844442)

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Immunoprecipitation

1:100-1:500

In vitro assay

Reported in scientific literature (PMID: 24844442)

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SETDB1

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described.

Alternate Names

ERG-associated protein with SET domain, ESETEC 2.1.1.43, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, histone-lysine N-methyltransferase SETDB1, histone-lysine N-methyltransferase, H3lysine-9 specific 4, KG1T, KIAA0067H3-K9-HMTase4, KMT1EERG-associated protein with a SET domain, ESET, Lysine N-methyltransferase 1E, SET domain bifurcated 1, SET domain, bifurcated 1

Gene Symbol

SETDB1

UniProt

Q15047 (Human)

Additional SETDB1 Products

Product Documents for SETDB1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SETDB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for SETDB1 Antibody

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Protocols

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FAQs

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