Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Orangutan (100%), Porcine (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 198 and 248 of human alternative splicing factor (splicing factor, arginine/serine-rich 1) using the numbering given in entry NP_008855.1 (GeneID 6426).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SF2 Antibody - BSA Free

Western Blot: SF2 Antibody [NBP1-19094]

Western Blot: SF2 Antibody [NBP1-19094]

Western Blot: SF2 Antibody [NBP1-19094] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IPloaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ASF antibody used for WB at 1 ug/ml (A and B) and used for IP at 10 ug/mg lysate.ASF was also immunoprecipitated by rabbit anti-ASF antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposuretimes of 10 seconds (A) and 30 seconds (B).

Applications for SF2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

10 ug/mg lysate

Western Blot

1:1000-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SF2

This gene encodes a member of the arginine/serine-rich splicing factor protein family, and functions in both constitutive and alternative pre-mRNA splicing. The protein binds to pre-mRNA transcripts and components of the spliceosome, and can either activate or repress splicing depending on the location of the pre-mRNA binding site. The protein's ability to activate splicing is regulated by phosphorylation and interactions with other splicing factor associated proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

Alternative-splicing factor 1, ASFpre-mRNA-splicing factor SF2, P33 subunit, MGC5228, serine/arginine-rich splicing factor 1, SF2FLJ53078, SF2p33, SFRS1splicing factor 2, Splicing factor, arginine/serine-rich 1ASF-1, SR splicing factor 1, SRp30a

Entrez Gene IDs

6426 (Human)

Gene Symbol

SRSF1

UniProt

Q07955 (Human)

Additional SF2 Products

Product Documents for SF2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SF2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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