SF20/MYDGF Antibody (2D4) - Azide and BSA Free

Novus Biologicals | Catalog # H00056005-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2D4

Format

Azide and BSA Free
View all SF20/MYDGF Primary Antibodies »

Product Specifications

Immunogen

SF20/MYDGF (AAH10129, 1 a.a. ~ 173 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAPSGGWNGVGASLWAALLLGAVALRPAEAVSEPTTVAFDVRPGGVVHSFSHNVGPGDKYTCMFTYASQGGTNEQWQMSLGTSEDHQHFTCTIWRPQGKSYLYFTQFKAEVRGAEIEYAMAYSKAAFERESDVPLKTEEFEVTKTAVAHRPGAFKAELSKLVIVAKASRTEL

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SF20/MYDGF Antibody (2D4) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: SF20/MYDGF Antibody (2D4) [H00056005-M03]

Immunocytochemistry/ Immunofluorescence: SF20/MYDGF Antibody (2D4) [H00056005-M03]

Immunocytochemistry/Immunofluorescence: SF20/MYDGF Antibody (2D4) [H00056005-M03] - Analysis of monoclonal antibody to SF20/MYDGF on HeLa cell. Antibody concentration 10 ug/ml
ELISA: SF20/MYDGF Antibody (2D4) [H00056005-M03]

ELISA: SF20/MYDGF Antibody (2D4) [H00056005-M03]

ELISA: SF20/MYDGF Antibody (2D4) [H00056005-M03] - Detection limit for recombinant GST tagged SF20/MYDGF is approximately 1ng/ml as a capture antibody.

Applications for SF20/MYDGF Antibody (2D4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1ng/mL

Immunocytochemistry/ Immunofluorescence

10ug/mL
Application Notes
Antibody reactive against recombinant protein on ELISA and Immunoflourescence. GST alone used as a negative control.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SF20/MYDGF

Myeloid-Derived Growth Factor, or MYDGF, is a Bone marrow-derived monocyte protein, and it is correlated with enhanced metabolic activity, suppression of apoptosis, and stimulation of cell proliferation (1). MYDGF is expressed predominantly in inflammatory cells, such as monocytes and macrophages (1). Up-regulation of MYDGF expression was also found during adipocyte differentiation (2). Expression of MYDGF was induced in the circulation and heart tissue after myocardial infarction. It promotes cardiac myocyte survival by stimulating endothelial cell proliferation through a MAPK1/3-, STAT3- and CCND1-mediated signaling pathway, and inhibits cardiac myocyte apoptosis in a PI3K/AKT-dependent signaling pathway (1). MYDGF was found over-expressed in approximately two-thirds of Hepatocellular Carcinoma (HCC) tissues, and its expression was significantly positively correlated with that of alpha-fetoprotein (AFP) (3). In HCC, MYDGF could regulate cell proliferation through activating Akt/mitogen-activated protein kinase pathways (3). Mouse MYDGF shares 92% amino acid sequence identity with both human and rat MYDGF. Intriguingly, virtually all homologs of MYDGF have a C-terminal putative ER retention sequence BXEL (B: Arg, His, or Lys; X: variable residue; E: Glu; L: Leu), which has the potential to retain human MYDGF and its homologs in the ER, whereas truncated MYDGF without BXEL is secreted from the cell (4). However, the functions of these different forms remain unclear.

Long Name

Stromal Cell-derived Growth Factor SF20

Alternate Names

C19orf10, Ly6elg, MYDGF

Entrez Gene IDs

56005 (Human)

Gene Symbol

MYDGF

OMIM

606746 (Human)

UniProt

AAH10129 (Human)

Additional SF20/MYDGF Products

Product Documents for SF20/MYDGF Antibody (2D4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SF20/MYDGF Antibody (2D4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for SF20/MYDGF Antibody (2D4) - Azide and BSA Free

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  • Q: Can IL 25 antibody be used in IP methods?

    A: H00056005-M03 has only been validated for ELISA and Immunoflourescence in Human. If you would be interested in using this antibody for IP I invite you to read about our Innovators Reward Program.

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