sFRP-4 Antibody (5#) - BSA Free
Novus Biologicals | Catalog # NBP3-41642
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Key Product Details
Species Reactivity
Human, Porcine
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 5#
Format
BSA Free
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Product Specifications
Immunogen
Immunized with recombinant Human sFRP-4 (Uniprot#: Q6FHJ7; Met265-Val346 (Uniprot#: Q6FHJ7)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for sFRP-4 Antibody (5#) - BSA Free
Immunohistochemistry-Paraffin: sFRP-4 Antibody (5#) [NBP3-41642] -
Immunohistochemistry-Paraffin: sFRP-4 Antibody (5#) [NBP3-41642] - DAB staining on IHC-P; Sample: Human Colon Tissue; Primary Ab: 10ug/ml NBP3-41642; Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] -
Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] - Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Porcine Heart lysate; Lane4: SKOV3 cell lysate Primary Ab: 2ug/ml NBP3-41642; Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] -
Western Blot: sFRP-4 Antibody (5#) [NBP3-41642] - Sample: Recombinant sFRP-4, Human.Applications for sFRP-4 Antibody (5#) - BSA Free
Application
Recommended Usage
Immunohistochemistry
5-20ug/mL
Immunohistochemistry-Paraffin
5-20ug/mL
Western Blot
0.5-2ug/mL
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
0.01M PBS, pH7.4, 50% glycerol
Format
BSA Free
Preservative
0.05% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: sFRP-4
Long Name
Secreted Frizzled Related Protein 4
Alternate Names
DDC-4, FRP-AP, frpHE, FrzB2, sFRP4
Gene Symbol
SFRP4
Additional sFRP-4 Products
Product Documents for sFRP-4 Antibody (5#) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for sFRP-4 Antibody (5#) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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