SFXN2 Antibody - BSA Free

Immunohistochemistry-Paraffin: SFXN2 Antibody - BSA Free [NBP1-85960]

Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunocytochemistry/ Immunofluorescence: SFXN2 Antibody - BSA Free [NBP1-85960]

Staining of human cell line U-2 OS shows localization to mitochondria.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960]

Analysis in control (vector only transfected HEK293T lysate) and SFXN2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Parkin mediates SFXN2 degradation through K48-linked polyubiquitination (A,B) Western blot (WB) analysis of SFXN2, Parkin, and Ubiquitin protein levels in HEK293 cells expressing Parkin-Myc, SFXN2-HA, and Ubiquitin variant (control vector, HA-Ub-WT, HA-Ub-K48, HA-Ub-K48R, or HA-Ub-KR). Anti-alpha -Tubulin was used as the loading control for the immunoblotting (IB, A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2, Parkin, and Ubiquitin protein levels in HEK293 cells expressing Parkin-Myc, SFXN2-HA, and Ubiquitin variant (control vector, HA-Ub-WT, HA-Ub-K63, HA-Ub-K63R, or HA-Ub-KR). Anti-alpha -Tubulin was used as the loading control for the IB (C), Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). Images are representative of at least three independent experiments that gave similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Parkin negatively regulates SFXN2 protein levels in cells (A,B) Western blot (WB) analysis of SFXN2 and Parkin protein levels in HEK293 cells transfected with scrambled siRNA or siRNA targeting Parkin (A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells overexpressing Parkin-Myc or control vector (C). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). (E,F) WB analysis of endogenous SFXN2 and Parkin protein levels in HEK293 cells overexpressing control vector or Parkin-Myc (E), with or without 10 μM MG132 treatment for 8 h before harvest. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (F). (G,H) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells overexpressing SFXN2-HA alone or Parkin-Myc plus SFXN2-HA (G), with or without 10 μM MG132 treatment for 8 h before harvest. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (H). (I,J) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells expressing SFXN2-HA and Parkin variant (control vector, Parkin-WT, Pakin-T240R, or Parkin-R42P). Anti-alpha -Tubulin was used as the loading control for the IB. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (J). Images are representative of at least three independent experiments that gave similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one-way ANOVA and two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Parkin negatively regulates SFXN2 protein levels in cells (A,B) Western blot (WB) analysis of SFXN2 and Parkin protein levels in HEK293 cells transfected with scrambled siRNA or siRNA targeting Parkin (A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells overexpressing Parkin-Myc or control vector (C). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). (E,F) WB analysis of endogenous SFXN2 and Parkin protein levels in HEK293 cells overexpressing control vector or Parkin-Myc (E), with or without 10 μM MG132 treatment for 8 h before harvest. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (F). (G,H) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells overexpressing SFXN2-HA alone or Parkin-Myc plus SFXN2-HA (G), with or without 10 μM MG132 treatment for 8 h before harvest. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (H). (I,J) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells expressing SFXN2-HA and Parkin variant (control vector, Parkin-WT, Pakin-T240R, or Parkin-R42P). Anti-alpha -Tubulin was used as the loading control for the IB. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (J). Images are representative of at least three independent experiments that gave similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one-way ANOVA and two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Parkin negatively regulates SFXN2 protein levels in cells (A,B) Western blot (WB) analysis of SFXN2 and Parkin protein levels in HEK293 cells transfected with scrambled siRNA or siRNA targeting Parkin (A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells overexpressing Parkin-Myc or control vector (C). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). (E,F) WB analysis of endogenous SFXN2 and Parkin protein levels in HEK293 cells overexpressing control vector or Parkin-Myc (E), with or without 10 μM MG132 treatment for 8 h before harvest. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (F). (G,H) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells overexpressing SFXN2-HA alone or Parkin-Myc plus SFXN2-HA (G), with or without 10 μM MG132 treatment for 8 h before harvest. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (H). (I,J) WB analysis of SFXN2 and Parkin protein levels in HEK293 cells expressing SFXN2-HA and Parkin variant (control vector, Parkin-WT, Pakin-T240R, or Parkin-R42P). Anti-alpha -Tubulin was used as the loading control for the IB. Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (J). Images are representative of at least three independent experiments that gave similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one-way ANOVA and two-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Mitochondrial impairment decreases SFXN2 levels (A,B) Western blot (WB) analysis of SFXN2 and PINK1 protein levels in HEK293 cells treated with 20 μM CCCP for the indicated time periods (A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2 and Cytochrome C (Cyt C) protein levels in HEK293 cells treated with the indicated concentrations of MPP+ for 24 h (C). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). (E,F) WB analysis of SFXN2 and PINK1 protein levels in HEK293 cells treated with vehicle control (0.05% (v/v) DMSO), CCCP (10 μM) alone, or CCCP (10 μM) combined with MG132 (10 μM) for 12 h (E). Quantification of relative SFXN2 protein levels normalized to actin is presented in the histogram (F). Images are representative of at least three independent experiments with similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Mitochondrial impairment decreases SFXN2 levels (A,B) Western blot (WB) analysis of SFXN2 and PINK1 protein levels in HEK293 cells treated with 20 μM CCCP for the indicated time periods (A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2 and Cytochrome C (Cyt C) protein levels in HEK293 cells treated with the indicated concentrations of MPP+ for 24 h (C). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). (E,F) WB analysis of SFXN2 and PINK1 protein levels in HEK293 cells treated with vehicle control (0.05% (v/v) DMSO), CCCP (10 μM) alone, or CCCP (10 μM) combined with MG132 (10 μM) for 12 h (E). Quantification of relative SFXN2 protein levels normalized to actin is presented in the histogram (F). Images are representative of at least three independent experiments with similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: SFXN2 Antibody - BSA Free [NBP1-85960] -

Parkin mediates SFXN2 degradation through K48-linked polyubiquitination (A,B) Western blot (WB) analysis of SFXN2, Parkin, and Ubiquitin protein levels in HEK293 cells expressing Parkin-Myc, SFXN2-HA, and Ubiquitin variant (control vector, HA-Ub-WT, HA-Ub-K48, HA-Ub-K48R, or HA-Ub-KR). Anti-alpha -Tubulin was used as the loading control for the immunoblotting (IB, A). Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (B). (C,D) WB analysis of SFXN2, Parkin, and Ubiquitin protein levels in HEK293 cells expressing Parkin-Myc, SFXN2-HA, and Ubiquitin variant (control vector, HA-Ub-WT, HA-Ub-K63, HA-Ub-K63R, or HA-Ub-KR). Anti-alpha -Tubulin was used as the loading control for the IB (C), Quantification of relative SFXN2 protein levels normalized to alpha -Tubulin is presented in the histogram (D). Images are representative of at least three independent experiments that gave similar results. Histogram data are presented as mean +/- SEM (N = 3). Statistical significance was analyzed using one way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001, ns, non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40894005), licensed under a CC-BY license. Not internally tested by Novus Biologicals.