SGK2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86102

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EVLRKEPYDRAVDWWCLGAVLYEMLHGLPPFYSQDVSQMYENILHQPLQIPGGRTVAACDLLQSLLHKDQRQRLGSKADFLEIKNHVFFSPINWDDLYHKRLTPPFNPNVTGPADLKHFDPEFTQEAVSKSIGCTPD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SGK2 Antibody - BSA Free

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102] - Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102]

Immunohistochemistry-Paraffin: SGK2 Antibody [NBP1-86102] - Staining of human tonsil shows no cytoplasmic positivity in non-germinal center cells as expected.
SGK2 Antibody - BSA Free Western Blot: SGK2 Antibody - BSA Free [NBP1-86102]

Western Blot: SGK2 Antibody - BSA Free [NBP1-86102]

Lane 1: Mouse liver tissue lysate
Lane 2: Rat liver tissue lysate
SGK2 Antibody - BSA Free Western Blot: SGK2 Antibody - BSA Free [NBP1-86102]

Western Blot: SGK2 Antibody - BSA Free [NBP1-86102]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Lane 4: Human plasma (IgG/HSA depleted)
Lane 5: Human liver tissue

Applications for SGK2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SGK2

SGK2 encodes a serine/threonine protein kinase. Although this gene product is similar to serum- and glucocorticoid-induced protein kinase (SGK), this gene is not induced by serum or glucocorticoids. This gene is induced in response to signals that activate phosphatidylinositol 3-kinase, which is also true for SGK. Two alternate transcripts encoding two different isoforms have been described.

Long Name

Serine/Threonine Kinase 2

Alternate Names

H-SGK2, Sgkl

Gene Symbol

SGK2

Additional SGK2 Products

Product Documents for SGK2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SGK2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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