SH3BP2 [p Ser427] Antibody - BSA Free

Novus Biologicals | Catalog # NB600-492

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SH3BP2 Primary Antibodies »

Product Specifications

Immunogen

This affinity purified SH3BP2 [p Ser427] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP2 protein (SH3 Domain Binding Protein 2). (Uniprot: P78314)

Reactivity Notes

This product is phospho specific for human SH3BP2.

Modification

p Ser427

Specificity

This product is an affinity purified antibody produced by immunoaffinity chromatography and is phospho specific for human SH3BP2.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This product is an affinity purified antibody produced by immunoaffinity chromatography and is phospho specific for human pS427.

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for SH3BP2 [p Ser427] Antibody - BSA Free

Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]

Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492]

Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492] - Detection of endogenous protein present in unstimulated human whole cell lysates). The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of 60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.
SH3BP2 [p Ser427] Antibody

SH3BP2 [p Ser427] Antibody

Western blot analysis is shown using Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.

Applications for SH3BP2 [p Ser427] Antibody - BSA Free

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunoprecipitation

1:100

Western Blot

1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SH3BP2 by western blotting in the appropriate cell lysate or extract.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: SH3BP2

SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. It also binds to phosphatidylinositols, thereby linking the hemopoietic tyrosine kinase, fes, to the cytoplasmic membrane in a phosphorylation dependent mechanism. Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).

Alternate Names

Abl-SH3 binding protein 2, Cherubism, CRBM, CRPM, FLJ42079, FLJ54978,3BP-2,3BP2, RES4-23, SH3 domain-binding protein 2, SH3-domain binding protein 2, TNFAIP3 interacting protein 2

Gene Symbol

SH3BP2

UniProt

P78314 (Human)

Additional SH3BP2 Products

Product Documents for SH3BP2 [p Ser427] Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SH3BP2 [p Ser427] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SH3BP2 [p Ser427] Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SH3BP2 [p Ser427] Antibody - BSA Free and earn rewards!

Have you used SH3BP2 [p Ser427] Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies