Shigatoxin 1 Antibody (13C4) - Azide Free

Novus Biologicals | Catalog # NBP3-11250

Novus Biologicals
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Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, Immunoassay, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 13C4

Format

Azide Free
View all Shigatoxin 1 Primary Antibodies »

Product Specifications

Immunogen

The monoclonal antibody 13C4 recognizes the 1B subunit of Shigatoxin 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Shigatoxin 1 Antibody (13C4) - Azide Free (NBP3-11250) is a monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for Shigatoxin 1 Antibody (13C4) - Azide Free

Application
Recommended Usage

Functional

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of toxin to be inactivated.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Format

Azide Free

Preservative

No Preservative

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Shigatoxin 1

The monoclonal antibody 13C4 recognizes the 1B subunit of Shiga-like toxin 1. Shiga-like toxins (SLTs), are also called Verotoxins. Enterohemorrhagic Escherichia coli (EHEC) strains which are primarily of serotypes 0157:H7, 026:H11 and O111:H8 have been incriminated as etiologic agents of hemorrhagic colitis and Hemolytic-uremic syndrome, a generalized disease characterized by acute renal failure, thrombocytopenia, and microangiopathic hemolytic anemia. There are several distinct E.coli SLTs. SLT-I and SLT-II are produced by EHEC. SLT-I and Shiga toxin share;99% deduced amino acid sequence homology, whereas SLT-I and SLT-II share about 60% deduced amino acid sequence homology. SLT-I and SLT-II are antigenically distinct. The protein structure of the toxin consists of two domains: the A polypeptide that inhibits protein synthesis by targeting ribosomes, and the B polypeptide pentamer that binds to the eukaryotic cell receptor globotriaosylceramide (Gb3) leading to receptor-mediated endocytosis.

Alternate Names

Shiga toxin 1 subunit B, Shiga-like toxin 1, Stx1, STX1B, Verotoxin 1, VT-1

Gene Symbol

stx1B

Additional Shigatoxin 1 Products

Product Documents for Shigatoxin 1 Antibody (13C4) - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Shigatoxin 1 Antibody (13C4) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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