SHOX2 Antibody (1D1) - Azide and BSA Free

Novus Biologicals | Catalog # H00006474-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D1

Format

Azide and BSA Free
View all SHOX2 Primary Antibodies »

Product Specifications

Immunogen

SHOX2 (NP_006875, 117 a.a. ~ 204 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SPELKDRKDDAKGMEDEGQTKIKQRRSRTNFTLEQLNELERLFDETHYPDAFMREELSQRLGLSEARVQVWFQNRRAKCRKQENQLHK

Localization

Nuclear

Specificity

SHOX2 - short stature homeobox 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SHOX2 Antibody (1D1) - Azide and BSA Free

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01]

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01]

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01] - SHOX2 monoclonal antibody (M01), clone 1D1 Analysis of SHOX2 expression in PC-12.
Western Blot: SHOX2 Antibody (1D1) [H00006474-M01]

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01]

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01] - Analysis of SHOX2 over-expressed 293 cell line, cotransfected with SHOX2 Validated Chimera RNAi ( Cat # H00006474-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SHOX2 monoclonal antibody (M01), clone 1D1 (Cat # H00006474-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: SHOX2 Antibody (1D1) [H00006474-M01]

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01]

Western Blot: SHOX2 Antibody (1D1) [H00006474-M01] - Analysis of SHOX2 expression in transfected 293T cell line by SHOX2 monoclonal antibody (M01), clone 1D1.Lane 1: SHOX2 transfected lysate(37.6 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SHOX2 Antibody (1D1) [H00006474-M01] - Detection limit for recombinant GST tagged SHOX2 is 1 ng/ml as a capture antibody.

Applications for SHOX2 Antibody (1D1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SHOX2

This gene is a member of the homeo box family of genes that encode proteins containing a 60-amino acid residue motif that represents a DNA binding domain. Homeo box genes have been characterized extensively as transcriptional regulators involved in pattern formation in both invertebrate and vertebrate species. Several human genetic disorders are caused by aberrations in human homeo box genes. SHOX is a pseudoautosomal homeo box gene that is thought to be responsible for idiopathic short stature and implicated to play a role in the short stature phenotype of Turner syndrome patients. This gene is considered to be a candidate gene for Cornelia de Lange syndrome. Alternative splicing has been observed at this locus and two variants, each encoding a distinct isoform, have been identified.

Alternate Names

Homeobox protein Og12X, OG12SHOX homologous gene on chromosome 3, OG12Xshort stature homeobox protein 2, short stature homeobox 2, SHOTPaired-related homeobox protein SHOT

Entrez Gene IDs

6474 (Human)

Gene Symbol

SHOX2

OMIM

602504 (Human)

UniProt

NP_006875 (Human)

Additional SHOX2 Products

Product Documents for SHOX2 Antibody (1D1) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SHOX2 Antibody (1D1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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