SHP-1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-32179

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SHP-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human SHP-1. The exact sequence is proprietary.

Reactivity Notes

Chicken (80%).

Localization

Cytoplasm, Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SHP-1 Antibody - BSA Free

Western Blot: SHP-1 Antibody [NBP1-32179]

Western Blot: SHP-1 Antibody [NBP1-32179]

Western Blot: PTPN6 Antibody [NBP1-32179] - Sample (30 ug of whole cell lysate) A:NIH-3T37C 5% SDS PAGE; antibody diluted at 1:1000.
Immunoprecipitation: SHP-1 Antibody [NBP1-32179]

Immunoprecipitation: SHP-1 Antibody [NBP1-32179]

Immunoprecipitation: PTPN6 Antibody [NBP1-32179] - HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of SHP1 protein expressing HeLa cells B : Control with 3 ug of pre-immune rabbit IgG C : Immunoprecipitation of SHP1 by 3 ug of SHP1 7.5% SDS-PAGE The immunoprecipitated SHP1 protein was detected by SHP1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
SHP-1 Antibody

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] -

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP-1 Antibody

Western Blot: SHP-1 Antibody [NBP1-32179] -

Western Blot: SHP-1 Antibody [NBP1-32179] - Various whole cell extracts (10 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP-1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SHP-1 Antibody

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] -

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP-1 Antibody

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] -

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP-1 Antibody

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] -

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP-1 Antibody

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] -

Immunohistochemistry-Paraffin: SHP-1 Antibody [NBP1-32179] - SHP-1 antibody detects SHP-1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
SHP-1 stained by SHP-1 antibody (NBP1-32179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
SHP-1 Antibody

Western Blot: SHP-1 Antibody [NBP1-32179] -

Western Blot: SHP-1 Antibody [NBP1-32179] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP-1 antibody (NBP1-32179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SHP-1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SHP-1

SHP-1 (also called PTP1C, SH-PP1, HCP, SHP and PTPN6) is a protein tyrosine phosphatase (PTP) predominantly expressed in hematopoietic cells, and regulates multiple signaling pathways involved in hematopoietic cell growth, differentiation and activation (1). After cell stimulation, SHP-1 (as well as SHP-2), translocate from cytosol to plasma membrane and bind to the tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). SHP-1 has been involved in the negative regulation of various growth promoting receptors including receptor tyrosine kinase (c-Kit, CSF-1, EGF), cytokine receptors (IL-3, IFN-a/b) and other tyrosine based receptor CD22, B & T cell Ag receptor, Lck, Zap-70 and Vav (3-5).

Long Name

Src Homology Region 2 Domain Phosphatase 1

Alternate Names

HCPH, PTP1C, PTPN6, SHP1

Gene Symbol

PTPN6

Additional SHP-1 Products

Product Documents for SHP-1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SHP-1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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