SHP-2/PTPN11 [p Tyr542] Antibody (2E2) - BSA Free

Name: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26397
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26397

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E2 expressed in HEK293

Format

BSA Free

View all SHP-2 Primary Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human SHP-2/PTPN11 [p Tyr542] [UniProt Q06124]

Modification

p Tyr 542

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SHP-2/PTPN11 [p Tyr542] Antibody (2E2) - BSA Free

Immunoprecipitation: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] -

Immunoprecipitation: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Immunoprecipitating SHP-2/PTPN11 [p Tyr542] in Hela whole cell lysate treated with Pervanadate.
Lane 1: Rabbit control IgG (1ug) instead of NBP3-26397 in Hela whole cell lysate treated with Pervanadate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000).
Lane 2: NBP3-26397 (3ug) + Hela whole cell lysate treated with Pervanadate (1mg).
Lane 3: Hela whole cell lysate treated with Pervanadate (20ug).

Western Blot: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] -

Western Blot: SHP-2/PTPN11 [p Tyr542] Antibody (2E2) [NBP3-26397] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Pervanadate or not).
All lanes: SHP-2/PTPN11 [p Tyr542] Antibody at 0.65ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 68 KDa
Observed band size: 68 KDa

Applications for SHP-2/PTPN11 [p Tyr542] Antibody (2E2) - BSA Free

Application

Recommended Usage

Immunoprecipitation

1:200-1:1000

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SHP-2

SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Over expression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).

Long Name

Src Homology Region 2 Domain Phosphatase 2

Alternate Names

PTP1D, PTP2C, PTPN11, SH-PTP2, SHP2

Gene Symbol

PTPN11

Additional SHP-2 Products

Product Documents for SHP-2/PTPN11 [p Tyr542] Antibody (2E2) - BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SHP-2/PTPN11 [p Tyr542] Antibody (2E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.