SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free

Novus Biologicals | Catalog # NLS5411

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Hamster, Monkey, Primate, Rabbit

Cited:

Rat

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SHP/NR0B2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 16 amino acid peptide from ligand-binding domain of human SHP/NR0B2/Nuclear Receptor SHP.

Epitope

Ligand-binding domain

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 18586688). Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Equine (88%), Porcine (88%), Opossum (88%), Canine (81%).

Specificity

Human SHP/SHP/NR0B2/Nuclear Receptor SHP/Nuclear Receptor SHP. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody [NLS5411] - Breast, carcinoma
Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody [NLS5411] - Analysis of anti-NR0B2 antibody with human liver, hepatocytes.
Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody [NLS5411] - Pancreas, carcinoma
Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free [NLS5411]

Immunohistochemistry-Paraffin: SHP/NR0B2/Nuclear Receptor SHP Antibody [NLS5411] - Ovary, carcinoma

Applications for SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

4 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: SHP/NR0B2

SHP (short heterodimer partner) is a bile acid-dependent orphan NR0 Knirps-Like receptor with dimerization and ligand-binding domains but not the conventional DNA-binding domain. SHP has been shown to inhibit the transcriptional activity of other nuclear receptors, including thyroid hormone receptor, constitutive androstane receptor, and retinoic acid receptors. SHP affects hepatic cholesterol catabolism based on a two-step mechanism dependent on both coactivator competition and direct transcriptional repression by mediating the repression of CYP7A1, the rate-limiting enzyme for bile acid synthesis. Mutations in the SHP gene contribute to increased body weight, indicating a possible role of SHP in the development of early-onset diabetes (maturity-onset diabetes of the young, MODY). Loss of SHP in mice caused abnormal accumulation and increased synthesis of bile acids due to derepression of rate-limiting CYP7A1 and CYP8B1 hydroxylase enzymes in the biosynthetic pathway. SHP expression has been documented in human liver, adrenal, spleen, and pancreas. ESTs have been isolated from cancerous human kidney, lung, and stomach, as well as normal human heart, kidney, liver, and liver/spleen. Caution: The nuclear receptor SHP should not be confused with the tyrosine phosphatases SHP-1 and SHP-2.

Long Name

Small Heterodimer Partner

Alternate Names

NR0B2

Entrez Gene IDs

8431 (Human)

Gene Symbol

NR0B2

UniProt

Q15466 (Human)

Additional SHP/NR0B2 Products

Product Documents for SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free

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Protocols

View specific protocols for SHP/NR0B2/Nuclear Receptor SHP Antibody - BSA Free (NLS5411):

Immunohistochemistry Protocol for Nuclear Receptor SHP Antibody (NLS5411):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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