Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20963

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # BLCAM/8113R

Format

Azide and BSA Free
View all Siglec-2/CD22 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa1-200) of human Siglec-2/CD22 protein (exact sequence is proprietary)

Localization

Cell surface.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Raji cells or human peripheral blood lymphocytes (hPBL). Human tonsil, lymph node or spleen.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human tonsil stained with CD22 Recombinant Rabbit Monoclonal Antibody (BLCAM/8113R). Inset: PBS instead of primary antibody, secondary control.

Applications for Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Siglec-2/CD22

CD22 is a single-pass type 1 membrane glycoprotein found in the cytoplasm and cell membrane of B-lymphocytes. CD22 has at least two isoforms (alpha and beta) produced by alternate splicing. The protein exists predominantly as a monomer of the beta isoform (95 kDa). It is also found as a heterodimer of the beta and alpha isoforms.

Long Name

Sialic Acid Binding Ig-like Lectin 2

Alternate Names

BL-CAM, CD22, Siglec2

Gene Symbol

CD22

Additional Siglec-2/CD22 Products

Product Documents for Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Siglec-2/CD22 Antibody (BLCAM/8113R) - Azide and BSA Free

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  • Q: I am interested in buying a CD22 antibody for fluorescence microscopy and FACS I red on your web page that you also offer to label your antibodies, if required, is that correct?

    A: We do have the ability to perform custom conjugations on certain antibodies, including some to the CD22 protein. If you send me the conjugate you are interested in I can provide you with a quote.

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