Siglec-H Antibody (23M14A10) - BSA Free
Novus Biologicals | Catalog # NBP2-27061
![Western Blot: Siglec-H Antibody (23M14A10)BSA Free [NBP2-27061]](https://beta-resources.rndsystems.com/images/products/Siglec-H-Antibody-23M14A10-Western-Blot-NBP2-27061-img0001.jpg "Western Blot: Siglec-H Antibody (23M14A10)BSA Free [NBP2-27061]")
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2a Kappa Clone # 23M14A10
Format
BSA Free
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Product Specifications
Immunogen
SkRSHTkRVERPATHMDQA (aa286-304)
Clonality
Monoclonal
Host
Rat
Isotype
IgG2a Kappa
Description
Novus Biologicals Rat Siglec-H Antibody (23M14A10) - BSA Free (NBP2-27061) is a monoclonal antibody validated for use in IHC, WB and Flow. Anti-Siglec-H Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Siglec-H Antibody (23M14A10) - BSA Free
Western Blot: Siglec-H Antibody (23M14A10)BSA Free [NBP2-27061]
Western Blot: Siglec-H Antibody (23M14A10) [NBP2-27061] - WB analysis of mouse heart lysate using Siglec-H antibody (clone 23M14A10) at 3 ug/ml concentration.![Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]](https://beta-resources.rndsystems.com/images/products/Siglec-H-Antibody-23M14A10-Immunohistochemistry-Paraffin-NBP2-27061-img0006.jpg "Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]")
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse lung using 5 ug/ml concentration of SiglecH antibody (clone 23M14A10). Alkaline Phosphatase based detection was used for final staining development.![Flow Cytometry: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]](https://beta-resources.rndsystems.com/images/products/Siglec-H-Antibody-23M14A10-Flow-Cytometry-NBP2-27061-img0009.jpg "Flow Cytometry: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]")
Flow Cytometry: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]
Flow Cytometry: Siglec-H Antibody (23M14A10) [NBP2-27061] - Analysis using the PE conjugate of NBP2-27061. Staining of freshly isolated Balb C mouse splenocytes with B220 (clone RA3-6B2) Alexa Fluor 488 antibody and with Rat IgG2a PE (isotype control, left) or anti-mouse Siglec H PE antibody (Clone: 23M14A10, right).![Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]](https://beta-resources.rndsystems.com/images/products/Siglec-H-Antibody-23M14A10-Immunohistochemistry-Paraffin-NBP2-27061-img0004.jpg "Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]")
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse kidney using 5 ug/ml concentration of SiglecH antibody (clone 23M14A10).The cells of various renal tubules showed specific immunopositivity for SiglecH.![Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]](https://beta-resources.rndsystems.com/images/products/Siglec-H-Antibody-23M14A10-Immunohistochemistry-Paraffin-NBP2-27061-img0007.jpg "Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]")
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) - BSA Free [NBP2-27061]
Immunohistochemistry-Paraffin: Siglec-H Antibody (23M14A10) [NBP2-27061] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal mouse lung using 5 ug/ml concentration of SiglecH antibody (Clone 23M14A10). DAB based detection was used for final staining development.Applications for Siglec-H Antibody (23M14A10) - BSA Free
Application
Recommended Usage
Flow Cytometry
1ug/10^6cells~
Immunohistochemistry-Paraffin
5ug/ml
Western Blot
3-5ug/ml~
Application Notes
In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval. This antibody is CyTOF ready.
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Siglec-H
Long Name
Sialic Acid Binding Ig-like Lectin 7
Alternate Names
SiglecH
Gene Symbol
Siglech
Additional Siglec-H Products
Product Documents for Siglec-H Antibody (23M14A10) - BSA Free
Product Specific Notices for Siglec-H Antibody (23M14A10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Siglec-H Antibody (23M14A10) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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