SIM1 Antibody
Novus Biologicals | Catalog # NBP1-97773
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-SGDRYRTEQYQS corresponding to internal region according to NP_005059.2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for SIM1 Antibody
Western Blot: SIM1 Antibody [NBP1-97773]
Western Blot: SIM1 Antibody [NBP1-97773] - NBP1-97773 (1ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for SIM1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:2000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 80 kDa band observed in HEK293 lysates (calculated MW of 85.5 kDa band according to NP_005059.2).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: SIM1
Alternate Names
BHLHE14, bHLHe14single-minded homolog 1, Class E basic helix-loop-helix protein 14, single-minded (Drosophila) homolog 1, single-minded homolog 1 (Drosophila)
Gene Symbol
SIM1
UniProt
Additional SIM1 Products
Product Documents for SIM1 Antibody
Certificate of Analysis
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Product Specific Notices for SIM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SIM1 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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