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Simple Plex Human Adiponectin/Acrp30 Cartridge

Catalog #: SPCKB-PS-000337 Datasheet / COA / SDS
Simple Plex Human Adiponectin/Acrp30 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD09, and wash buffer
Catalog # Availability Size / Price Qty
SPCKB-PS-000337
SPCKA-PS-000337
SPCKC-PS-000337
Control Products Available
898067: Simple Plex Control for Human Adiponectin/Acrp30
Human total Adiponectin Simple Plex Assay
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Simple Plex Human Adiponectin/Acrp30 Cartridge Summary

Sample Type & Volume Required Per Well*
Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity
6.24 pg/mL
Assay Range
33.8 - 51,540 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Adiponectin.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Adiponectin in serum and plasma (EDTA/Heparin).

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 37 37
Mean (pg/mL) 336 15446 304 14616
Standard Deviation 29.9 1004 24.1 985
CV% 8.9 6.5 7.9 6.7

Recovery

Recovery was not necessary as Adiponectin shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human Adiponectin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human Adiponectin/Acrp30 Ella Assay Linearity

Scientific Data

N/A Human total Adiponectin Simple Plex Assay Standard Curve View Larger

Human Adiponectin/Acrp30 Ella Assay Standard Curve

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Product Datasheet
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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Adiponectin/Acrp30

Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.

Entrez Gene IDs:
9370 (Human); 11450 (Mouse); 246253 (Rat)
Alternate Names:
ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; Adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein

FAQs

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