Simple Plex Human ASC Cartridge

Catalog #: SPCKB-PS-000834 Datasheet / COA / SDS
Simple Plex Human ASC assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
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Human ASC Simple Plex Assay
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Simple Plex Human ASC Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (25 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL)
5.24 pg/mL
Assay Range
5.2 - 8,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Natural and recombinant human ASC.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human ASC in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin)


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 16 16
Mean (pg/mL) 65.2 3191 61.4 3062
Standard Deviation 3.21 170 6.23 294
CV% 4.9 5.3 10.1 9.6


Recovery at three different spiked concentrations within the range of the assay was evaluated.


Samples containing and/or spiked with high concentrations of human ASC were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human ASC Simple Plex Assay Linearity

Scientific Data

Human ASC Simple Plex Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ASC

ASC contains an N-terminal Pyrin domain and C-terminal CARD (Caspase-recruitment Domain), and is a p53 target protein that serves as an adaptor to the pro-apoptotic Bax molecule. The interaction of ASC with Bax promotes apoptosis in response to many apoptotic stimuli. Methylation of the ASC gene has been correlated with decreased protein expression in numerous types of cancer.

Long Name:
Apoptosis-Associated Speck-Like Protein Containing A CARD
Entrez Gene IDs:
29108 (Human)
Alternate Names:
apoptosis-associated speck-like protein containing a CARD; ASC; CARD5; caspase recruitment domain protein 5; Caspase recruitment domain-containing protein 5; hASC; PYCARD; PYD and CARD domain-containing protein; Target of methylation-induced silencing 1; TMS; TMS1; TMS-1


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