Simple Plex Human CD14 Cartridge

Name: Simple Plex Human CD14 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000386
Price: 600 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000386

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Product Documents

Simple Plex Human CD14 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)

Sensitivity

1 pg/mL

Assay Range

16.9-4130 pg/mL (Serum, EDTA Plasma, Heparin Plasma, Urine)

Simple Plex Human CD14 Cartridge Summary

Simple Plex assay is for the detection of human CD14 in serum, plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all CD14 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human CD14.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one CD14 assay.

Inter-Assay Precision (Precision between assays) Replicates of each CD14 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human CD14.

EDTA Plasma, Heparin Plasma, Serum, Urine

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	31	31
Mean (pg/mL)	31.7	1308	28.1	1233
Standard Deviation	3.07	95.9	2.74	162
CV%	9.7	7.3	9.7	13.2

Recovery for Simple Plex Human CD14 Cartridge

Recovery was not necessary as human CD14 shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human CD14 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human CD14 Cartridge

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human CD14 Assay. Assay range is 16.9-4130 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD14

CD14 is an acute phase glycoprotein that binds lipopolysaccharide endotoxins (LPS) from Gram-negative bacteria. It is primarily expressed on monocytes, macrophages, and neutrophils, and soluble forms can be secreted or shed from the cell surface. CD14 accepts LPS from its initial binding by LBP, and it can further transfer LPS to the TLR4/MD2 complex. LPS acctivation of TLR4 triggers inflammatory activation. CD14 also contributes to the response to TLR3 ligands, phosphoinositides, as well as the surfactant proteins SP-A and SP-D.