Simple Plex Human EGF Cartridge

R&D Systems | Catalog # SPCKB-PS-000311

Simple Plex Human EGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), Platelet-poor EDTA Plasma (25 µL), Platelet-poor Heparin Plasma (25 µL), Urine (10 µL)

Sensitivity

0.112 pg/mL

Assay Range

0.2-4680 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)

Simple Plex Human EGF Cartridge Summary

Simple Plex assay is for the detection of human Epidermal Growth Factor (EGF) in cell culture supernatant (CCS), serum, platelet-poor plasma (EDTA/Heparin), and urine in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all EGF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human EGF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one EGF assay.

Inter-Assay Precision (Precision between assays) Replicates of each EGF control were tested in multiple assays performed by at least three technicians using two lots of reagents for human EGF.

Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 9 9
Mean (pg/mL) 30.8 1355 30.1 1467
Standard Deviation 1.19 79.3 1.43 139
CV% 3.9 5.9 4.8 9.5

Recovery for Simple Plex Human EGF Cartridge

Recovery for the human EGF assays was evaluated at three different spiked concentrations of EGF protein.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 114 112-117
Platelet-poor EDTA Plasma (n=3) 92 74-103
Platelet-poor Heparin Plasma (n=3) 89 70-103
Serum (n=5) 93 83-107

Linearity

Samples containing and/or spiked with high concentrations of human EGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human EGF Assay Linearity

Scientific Data Images for Simple Plex Human EGF Cartridge

Simple Plex Human EGF Assay Standard Curve

Simple Plex Human EGF Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human EGF Assay. Assay range is 0.2-4680 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Long Name

Epidermal Growth Factor

Alternate Names

HOMG4, URG, Urogastrone

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Gene Symbol

EGF

UniProt

P01133

Additional EGF Products

Product Documents for Simple Plex Human EGF Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human EGF Cartridge

For research use only

Citations for Simple Plex Human EGF Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human EGF Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human EGF Cartridge and earn rewards!

Have you used Simple Plex Human EGF Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card