Simple Plex Human EGF Cartridge

Catalog #: SPCKB-PS-000311 Datasheet / COA / SDS
Simple Plex Human EGF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer
Catalog # Availability Size / Price Qty
Control Products Available
Human EGF Ella Assay
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Product Details
Supplemental Products

Simple Plex Human EGF Cartridge Summary

Sample Type & Volume Required Per Well*
Cell Culture Supernates (10 uL), Serum (25 uL), Platelet-poor EDTA Plasma (25 uL), Platelet-poor Heparin Plasma (25 uL), Urine (10 uL)
0.112 pg/mL
Assay Range
0.2 - 4,680 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine)
Natural and recombinant human EGF.
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay’s minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

Product Summary

Simple Plex assay for the detection of human Epidermal Growth Factor (EGF) in cell culture supernatant (CCS), serum, platelet-poor plasma (EDTA/Heparin), and urine.


Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 9 9
Mean (pg/mL) 30.8 1355 30.1 1467
Standard Deviation 1.19 79.3 1.43 139
CV% 3.9 5.9 4.8 9.5


Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=2) 114 112-117
Platelet-poor EDTA Plasma (n=3) 92 74-103
Platelet-poor Heparin Plasma (n=3) 89 70-103
Serum (n=5) 93 83-107


Samples containing and/or spiked with high concentrations of human EGF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Human EGF Ella Assay Linearity

Scientific Data

Human EGF Ella Assay Standard Curve

Product Datasheets

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Preparation and Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Long Name:
Epidermal Growth Factor
Entrez Gene IDs:
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names:
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone


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