Simple Plex Human Endoglin/CD105 Cartridge

Name: Simple Plex Human Endoglin/CD105 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000371
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000371

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Product Documents

Simple Plex Human Endoglin/CD105 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD06, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (5 µL), EDTA Plasma (5 µL), Heparin Plasma (5 µL)

Sensitivity

5.66 pg/mL

Assay Range

15.4-147150 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human Endoglin/CD105 Cartridge Summary

Simple Plex assay is for the detection of human Endoglin in serum and plasma (EDTA/Heparin) in Sample Diluent SD06.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human Endoglin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one Endoglin assay.

Inter-Assay Precision (Precision between assays) Replicates of each Endoglin control were tested in multiple assays performed by at least three technicians using two lots of reagents for human Endoglin.

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	16	17
Mean (pg/mL)	951	45,292	1001	49,876
Standard Deviation	90.5	1760	74.9	4456
CV%	9.5	3.9	7.5	8.9

Recovery for Simple Plex Human Endoglin/CD105 Cartridge

Recovery was not necessary as human Endoglin shows 5 points of natural linearity.

Linearity

Samples containing and/or spiked with high concentrations of human Endoglin were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human Endoglin/CD105 Cartridge

Simple Plex Human Endoglin Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human Endoglin Assay. Assay range is 15.4-147,150 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Endoglin/CD105

Endoglin (CD105) is a transmembrane type III receptor for TGF-beta superfamily ligands and plays an important role in smooth muscle differentiation, angiogenesis, and neovascularization. It is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. Human Endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I. Elevated levels of anti-angiogenic soluble Endoglin contribute to pathogenicity in preeclampsia. Endoglin associates with the receptors TGF-beta RII, Activin RIIA or RIIB, BMPR-IA/ALK-3, or BMPR-IB/ALK6 and enhance binding of Activin A, BMP-2, -7, -9, TGF-beta 1, or TGF-beta 3. Endoglin can either enhance or inhibit signaling through these receptor complexes.