Simple Plex Human GM-CSF Cartridge

Name: Simple Plex Human GM-CSF Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000493
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000493

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Product Documents

Simple Plex Human GM-CSF assay kit for use on Ella instrument. Contains cartridge, sample diluent SD11, SD12 and SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.56 pg/mL

Assay Range

0.7-6730 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human GM-CSF Cartridge Summary

Simple Plex assay for the detection of human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin).

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all GM-CSF Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human GM-CSF.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	11	10
Mean (pg/mL)	38.5	1739	38.0	1557
Standard Deviation	1.88	40.7	2.11	111
CV%	4.9	2.3	5.6	7.1

Recovery for Simple Plex Human GM-CSF Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	98	91-111
Heparin Plasma (n=4)	96	93-99
Serum (n=4)	94	82-107

Linearity

Samples containing and/or spiked with high concentrations of human GM-CSF were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human GM-CSF Cartridge

Human GM-CSF Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: GM-CSF

GM-CSF (Granulocyte-Macrophage Colony Stimulating Factor) induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor. The beta c subunit is shared by the receptor complexes for IL-3 and IL-5.