Simple Plex Human IFN-alpha (multi-subtype) Cartridge

Name: Simple Plex Human IFN-alpha (multi-subtype) Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-002812
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-002812

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Product Documents

Simple Plex Human IFN-alpha assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.51 pg/mL

Assay Range

1.46-13870 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IFN-alpha (multi-subtype) Cartridge Summary

Simple Plex assay is for the detection of human Interferon alpha (IFN-alpha ) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IFN-alpha (multi-subtype).

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IFN-alpha (multi-subtype) assay.

Inter-Assay Precision (Precision between assays) Replicates of each IFN-alpha (multi-subtype) control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IFN-alpha (multi-subtype).

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	46	48
Mean (pg/mL)	82.6	4775	84.2	4425
Standard Deviation	3.71	215	7.41	390
CV%	4.5	4.5	8.8	8.8

Recovery for Simple Plex Human IFN-alpha (multi-subtype) Cartridge

Recovery for the human IFN-alpha (multi-subtype) assays was evaluated at three different spiked concentrations of IFN-alpha (multi-subtype) protein.

Sample Type	Average % Recovery	Range %
EDTA Plasma (n=4)	88	83-91
Heparin Plasma (n=4)	86	80-92
Serum (n=4)	88	84-90

Linearity

Samples containing and/or spiked with high concentrations of human IFN-alpha (multi-subtype) were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human IFN-alpha (multi-subtype) Cartridge

Simple Plex Human IFN-alpha (multi-subtype) Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IFN-alpha (multi-subtype) Assay. Assay range is 1.46-13,870 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-alpha

Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.