Simple Plex Human IFN-beta Cartridge

Name: Simple Plex Human IFN-beta Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000934
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000934

Submit a review

Citations (1)

Product Documents

Simple Plex Human IFN-beta assay kit contains cartridge, sample diluent SD10, and wash buffer. Sample type indicated with PS (Plasma/Serum).

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.18 pg/mL (Cell Culture Supernates)
1.03 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Assay Range

1.18-1804 pg/mL (Cell Culture Supernates)
6.16-3760 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IFN-beta Cartridge Summary

Simple Plex assay for the detection of human IFN-beta in cell culture supernatant (CCS), serum, plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IFN-beta Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IFN-beta

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	29	28
Mean (pg/mL)	13.5	551	11.2	507
Standard Deviation	1.27	45.9	1.38	57.9
CV%	9.4	8.3	12.3	11.4

Recovery for Simple Plex Human IFN-beta Cartridge

Recovery at three different spiked concentrations within the range of the assay was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Supernates (n=4)	97	91-104
EDTA Plasma (n=4)	92	84-101
Heparin Plasma (n=4)	80	76-86
Serum (n=4)	95	83-106

Linearity

Samples containing and/or spiked with high concentrations of human IFN-beta were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human IFN-beta Cartridge

Human IFN-beta Ella Assay Standard Curve - CCS with Sample Diluent SD13

Human IFN-beta Ella Assay Standard Curve - Serum/Plasma with Sample Diluent SD10

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-beta

IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.