Simple Plex Human IL-11 Cartridge

R&D Systems | Catalog # SPCKB-PS-000648

Simple Plex Human IL-11 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

Customize This Cartridge

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

Customize This Cartridge

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (25 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

2 pg/mL

Assay Range

11.7-44600 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IL-11 Cartridge Summary

Simple Plex assay is for the detection of human Interleukin 11 (IL-11) in cell culture supernatant (CCS), serum, and plasma (EDTA/Heparin) in Sample Diluent SD13. 

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-11 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-11.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-11 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-11 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-11.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 64 64
Mean (pg/mL) 306 15,605 313 14,627
Standard Deviation 10.0 1173 22.1 1282
CV% 3.3 7.5 7.0 8.8

Recovery for Simple Plex Human IL-11 Cartridge

Recovery for the human IL-11 assays was evaluated at three different spiked concentrations of IL-11 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 94 88-101
Heparin Plasma (n=4) 94 83-104
Serum (n=4) 101 95-106

Linearity

Samples containing and/or spiked with high concentrations of human IL-11 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human IL-11 Assay Linearity

Scientific Data Images for Simple Plex Human IL-11 Cartridge

Simple Plex Human IL-11 Assay Standard Curve

Simple Plex Human IL-11 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-11 Assay. Assay range is 11.7-44,600 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-11

Interleukin-11, also known as AGIF, is a pleiotropic cytokine in the IL-6 family, which also includes LIF, CNTF, Oncostatin M, Cardiotrophin-1, IL-27, and IL-31. It is secreted by osteoblasts, synoviocytes, fibroblasts, chondrocytes, intestinal myofibroblasts, and trophoblasts. It is found in the plasma mainly during inflammation, and is considered to be primarily anti-inflammatory. It stimulates hematopoiesis and thrombopoiesis, regulates macrophage differentiation, and confers mucosal protection in the intestine. It has also been found to enhance T cell polarization toward Th2, promote B cell IgG production, increase osteoclast bone absorption, protect endothelial cells from oxidative stress, and regulate epithelial proliferation and apoptosis. IL-11 signals through a receptor complex that contains IL-11 R alpha and gp130. A soluble form of IL-11 R alpha can bind IL-11 and either form a signaling complex with gp130 on the cell surface or inhibit cell surface IL-11 R alpha/gp130 signaling.

Long Name

Interleukin 11

Alternate Names

AGIF, IL11, Oprelvekin

Entrez Gene IDs

3589 (Human); 16156 (Mouse); 171040 (Rat); 102128313 (Cynomolgus Monkey)

Gene Symbol

IL11

Additional IL-11 Products

Product Documents for Simple Plex Human IL-11 Cartridge

Loading product document information...

Loading product document information...

Certificate of Analysis

To download a Certificate of Analysis, please enter your Simple Plex catalog number and lot number in the search boxes below.

Product Specific Notices for Simple Plex Human IL-11 Cartridge

For research use only

Citations for Simple Plex Human IL-11 Cartridge

View all citations for the Simple Plex platform.

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Customer Reviews for Simple Plex Human IL-11 Cartridge

There are currently no reviews for this product. Be the first to review Simple Plex Human IL-11 Cartridge and earn rewards!

Have you used Simple Plex Human IL-11 Cartridge?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card