Simple Plex Human IL-21 Cartridge

Name: Simple Plex Human IL-21 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-013133
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-013133

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Product Documents

Simple Plex Human IL-21 assay kit for use on Ella instrument. Contains cartridge, Sample Diluent SD13, and Wash Buffer.

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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.

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Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Cell Culture Supernates (10 µL), Serum (25 µL), EDTA Plasma (25 µL)

Sensitivity

0.18 pg/mL

Assay Range

0.78-7455 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)

Simple Plex Human IL-21 Cartridge Summary

Simple Plex assay is for the detection of human Interleukin 21 (IL-21) in cell culture supernatant (CCS), serum, and EDTA plasma in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

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Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

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Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-21.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each IL-21 control was tested multiple times in one human IL-21 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-21 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-21.

Cell Culture Supernates, EDTA Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	52	51
Mean (pg/mL)	18.6	952	22.5	1046
Standard Deviation	0.964	69.2	2.42	77.0
CV%	5.2	7.3	10.8	7.4

Recovery for Simple Plex Human IL-21 Cartridge

Recovery for the human IL-21 assays was evaluated at three different spiked concentrations of IL-21 protein.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=2)	99	97-101
EDTA Plasma (n=4)	100	95-107
Serum (n=4)	112	104-122

Linearity

Samples containing and/or spiked with high concentrations of human IL-21 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay.

Scientific Data Images for Simple Plex Human IL-21 Cartridge

Simple Plex Human IL-21 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-21 Assay. Assay range is 0.78-7,455 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-21

IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.