Simple Plex Human IL-33 Cartridge

R&D Systems | Catalog # SPCKB-PS-000363

Simple Plex Human IL-33 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.
R&D Systems

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Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)

Sensitivity

0.452 pg/mL

Assay Range

0.75-1140 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human IL-33 Cartridge Summary

Simple Plex assay is for the detection of human Interleukin 33 (IL-33) in serum and plasma (EDTA/Heparin) in Sample Diluent SD13.

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all IL-33 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.
Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human IL-33.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested multiple times in one IL-33 assay.

Inter-Assay Precision (Precision between assays) Replicates of each IL-33 control were tested in multiple assays performed by at least three technicians using two lots of reagents for human IL-33.

EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 1 2
n 16 16 12 12
Mean (pg/mL) 6.85 326 6.67 328
Standard Deviation 0.314 17.1 0.320 13.5
CV% 4.6 5.3 4.8 4.1

Recovery for Simple Plex Human IL-33 Cartridge

Recovery for the human IL-33 assays was evaluated at three different spiked concentrations of IL-33 protein.

Sample Type Average % Recovery Range %
EDTA Plasma (n=4) 108 102-116
Heparin Plasma (n=4) 106 102-112
Serum (n=4) 110 100-121

Linearity

Samples containing and/or spiked with high concentrations of human IL-33 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Simple Plex Human IL-33 Assay Linearity

Scientific Data Images for Simple Plex Human IL-33 Cartridge

Simple Plex Human IL-33 Assay Standard Curve

Simple Plex Human IL-33 Assay Standard Curve

Data shown represents typical performance results for Lower Limit of Quantitation (LLOQ) and Upper Limit of Quantitation (ULOQ) for Simple Plex Human IL-33 Assay. Assay range is 0.75-1140 pg/mL.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-33

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

Long Name

Interleukin 33

Alternate Names

C9orf26, DVS27, IL33, NF-HEV

Entrez Gene IDs

90865 (Human); 77125 (Mouse)

Gene Symbol

IL33

Additional IL-33 Products

Product Documents for Simple Plex Human IL-33 Cartridge

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Certificate of Analysis

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Product Specific Notices for Simple Plex Human IL-33 Cartridge

For research use only

Citations for Simple Plex Human IL-33 Cartridge

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