Simple Plex Human PDGF-BB Cartridge
R&D Systems | Catalog # SPCKB-PS-000283
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Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize this cartridge.
Key Product Details
Assay Length
Sample Type & Volume Required Per Well*
Sensitivity
Assay Range
Simple Plex Human PDGF-BB Cartridge Summary
*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.
Compatible Instruments
Ella Automated Immunoassay System
Product Specifications
Assay Type
Format
Species
Specificity
Cross-reactivity
Interference
Precision
Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay.
Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents.
Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||
|---|---|---|---|---|
| Sample | 1 | 2 | 1 | 2 |
| n | 16 | 16 | 7 | 6 |
| Mean (pg/mL) | 7.20 | 294 | 6.92 | 297 |
| Standard Deviation | 0.390 | 15.7 | 0.392 | 18.8 |
| CV% | 5.4 | 5.3 | 5.7 | 6.3 |
Recovery for Simple Plex Human PDGF-BB Cartridge
Recovery was not necessary as PDGF-BB shows natural linearity data from a neat sample to a 1:16 dilution.
Linearity
Samples containing and/or spiked with high concentrations of human PDGF-BB were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay
Scientific Data Images for Simple Plex Human PDGF-BB Cartridge
Human PDGF-BB Simple Plex Assay Standard Curve
Preparation and Storage
Shipping
Stability & Storage
Background: PDGF-BB
PDGF-BB (Platelet-Derived Growth Factor BB) is a homodimeric molecule expressed by hepatocytes and nonresorbing osteoclasts, and it promotes osteoblast development and bone formation. It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization. The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta. PDGF-BB has been shown to bind the alpha/alpha homodimer, alpha/beta heterodimer, and the beta/beta homodimer in vitro, and act through the beta/beta homodimer in vivo.
Long Name
Alternate Names
Gene Symbol
Additional PDGF-BB Products
Product Documents for Simple Plex Human PDGF-BB Cartridge
Certificate of Analysis
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Product Specific Notices for Simple Plex Human PDGF-BB Cartridge
For research use only
Related Research Areas
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