Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Name: Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge
Brand: R&D Systems
SKU: SPCKB-PS-000383
Price: 450 USD
Availability: InStock

R&D Systems | Catalog # SPCKB-PS-000383

Submit a review

Citations

Product Documents

Simple Plex Human VEGFR2/KDR/Flk-1 assay kit for use on Ella instrument. Contains cartridge, sample diluent SD13, and wash buffer.

Customize My Cartridge on Bio-Techne

Simple Plex cartridges are custom built to fit your needs. Choose from over 350 assays spanning human, mouse, rat, and non-human primate. Just select your sample type, cartridge size (16, 32, or 72 samples), and up to 8 analytes per cartridge. Customize your cartridge on Bio-Techne.com

Customize My Cartridge

Compatible Instruments
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

90 minutes

Sample Type & Volume Required Per Well*

Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)

Sensitivity

14.9 pg/mL

Assay Range

52.3-31900 pg/mL (Serum, EDTA Plasma, Heparin Plasma)

Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge Summary

Simple Plex assay for the detection of human VEGF R2 in serum and plasma (EDTA/Heparin)

*The Sample Volume represented is based on the amount of sample incorporated into the reaction after taking into account the assay's minimum required dilution for a given matrix. Serial dilution may be necessary to achieve some of the final sample volumes represented.

View all VEGFR2/KDR/Flk-1 Simple Plex »

Compatible Instruments

Ella Automated Immunoassay System

Efficiently analyze biomarkers with Ella's advanced technology, providing accurate, precise results in under 90 minutes. Quantify up to 8 targets per assay through a streamlined automated workflow. Choose from >350 highly validated Simple Plex assays or Simple Plex discovery assays.

Learn More

Product Specifications

Assay Type

Automated sandwich ELISA

Format

Microfluidic cartridge containing three Glass Nano Reactors (GNR)

Species

Human

Specificity

Natural and recombinant human VEGF R2.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Each control was tested 16 times in one assay

Inter-Assay Precision (Precision between assays) Replicates of each control were tested in multiple assays performed by at least three technicians using two lots of reagents

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	16	16	7	8
Mean (pg/mL)	222	10,677	193	9248
Standard Deviation	12.6	529	24.3	944
CV%	5.6	5.0	12.6	10.2

Recovery for Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Recovery was not necessary as VEGF R2 shows natural linearity data from a neat sample to a 1:16 dilution.

Linearity

Samples containing and/or spiked with high concentrations of human VEGF R2 were serially diluted with Sample Diluent to produce samples within the dynamic range of the assay

Scientific Data Images for Simple Plex Human VEGFR2/KDR/Flk-1 Cartridge

Human VEGFR2/KDR/Flk-1 Ella Assay Standard Curve

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: VEGFR2/KDR/Flk-1

VEGFR2/KDR/Flk-1 (vascular endothelial growth factor receptor 2) is a transmembrane receptor tyrosine kinase that mediates the angiogenic effects of VEGF-A and VEGF-C. It is expressed primarily on vascular endothelial cells and endothelial cell progenitors. It is also expressed on endometrial epithelium, hematopoietic stem cells, liver sinusoidal endothelial cells, Sertoli cells and Leydig cells, platelets and megakaryocytes, sensory and autonomic neurons, Schwann cells, Muller glial cells, retinal progenitors, and osteoblasts. VEGFR2 can associate with VEGFR1, and a soluble form of VEGFR2 can circulate in the serum.